EFFECT OF 2,4-D LEVEL AND NODAL POSITION ON THE CALLUS INITIATION OF NODAL SEGMENTS OF BUCHLOE DACTYLOIDES (NUTT.) ENGELM.

in HortScience
View More View Less
  • 1 Department of Horticulture, University of Nebraska, Lincoln, NE 68583-0724

Stolon nodal segments of Buchloe dactyloides (Nutt.) Engelm. were removed from greenhouse grown plants and placed on Gamborg's B5 medium in order to determine nodal position and 2,4-D level required to give maximum callus initiation. 2,4-D levels used were 5uM, 16uM, 35uM, and 50uM. Six nodal segments were grouped according to position on the stolon, from the most recent node (node one) to the basal node (node 6). It was concluded that node 4 gave statistically greater callus mass than nodes 1, 2, 3, 5, and 6. Increasing levels of 2,4-D suppressed callus initiation, with maximum response occurring at 5uM 2,4-D.

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 41 14 3
PDF Downloads 75 37 1