HISTOLOGICAL EXAMINATION OF IN VITRO ADVENTITIOUS BUD DEVELOPMENT ON HYPOCOTYLS OF FRASER FIR

in HortScience
View More View Less
  • 1 Department of Horticultural Science, North Carolina State University, Raleigh, NC 27695
  • | 2 Department of Forestry, North Carolina State University, Raleigh, NC 27695

Hypocotyls of Fraser fir (Abies fraseri (Pursh) Poir.) were excised from seeds germination 9 days and placed on bud induction medium containing 10 mg/liter benzyladenine (BA) and 0.01 mg/liter naphthaleneacetic acid (NAA) or medium without growth regulators. After 3 days on medium containing growth regulators, cell divisions were localized in epidermal and subepidermal layers of the hypocotyl while similar cell divisions were not observed in control-treated hypocotyls. Cell clusters consisting of two to five cells were present after 7 days in hypocotyls placed on bud induction medium. In control-treated hypocotyls, stomata continued to develop and cells within the cortex became vacuolated during the first 2 weeks in culture. All hypocotyls were transferred to secondary medium after 3 weeks. Cell clusters continued to enlarge into meristemoids in hypocotyls initially placed on bud induction medium. Gradually, meristemoids developed into buds and cataphylls were observed covering bud meristems.

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 34 3 1
PDF Downloads 77 39 9