Petunia seeds of `White Cascade', `Red Flash', and `Red Madness' were sown in 406 plug trays on the same date. The first transplanting occurred when the plants could be removed from the cells without root damage. Subsequent transplanting occurred for four weeks. The first transplanting of `White Cascade' flowered two weeks earlier than the second while the third transplanting was one week behind the second. `Red Flash' flowered two weeks earlier for the first transplanting. There was no effect on time to flower for the `Red Madness'. The highest fresh and dry weights corresponded to the earliest flowering transplants. Optimum growth and development for most petunia cultivars was obtained with the earliest transplanting without root damage.