CHARACTERIZATION OF SOMACLONAL VARIATION IN TISSUE CULTURE-DERIVED TANGERINE-VIRESCENT LINES OF TOMATO

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  • 1 Department of Horticulture, Purdue University, West Lafayette, IN 47907

The tangerine-virescent (tv) mutation was reported as a single gene somaclonal variant from tissue culture (Evans and Sharp 1963). A replicated field trial was conducted to characterize variation and stability in the phenotype of this tv somaclone and to compare it with the inbred parent from which it was reportedly derived.

Heritability and stability of the tv somaclonal variant was measured by comparing R3 end R4 lines of sexual progenies of the original tv variant and with its sexually derived inbred parent UC82B. Several additional variants were observed in these tv lines, including fruit shape, days to first flower, fruit weight, yield, plant type, and fertility. Eight sterile or semi-sterile plants were discovered in 6 of 39 R4 lines. Our results suggest that multiple genetic changes have occurred in the tv somaclonal variant and while the original tv mutant is stably inherited, additional genetic abnormalities occur following sexual reproduction.

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