Cauliflower protoplasts with male fertile and Ogura male sterile cytoplasm were fused. Organelle reassortment and recombinant mitochondria were found in calli and plants regenerated from the fused protoplasts. Pretreatment (gamma-irradiation or iodoacetate) and protoplasm source (leaf or hypocotyl tissue) were manipulated in a series of fusions to determine their effects on organelle segregation. Some regenerated plants appear to combine Ogura male sterile mitochondria with normal Brassica chloroplasts. Plants with this organelle combination should be free of the cold temperature chlorosis due to incompatibility between the Brassica nucleus and the radish chloroplasts of the Ogura cytoplasm. These plants may have potential for improved cauliflower hybrid production.
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