ANALYSIS OF FRUIT BUD PROTEINS ASSOCIATED WITH PLANT DORMANCY

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  • 1 Department of Horticulture, Louisiana State University, Baton Rouge, LA 70803

Plant dormancy research has long been stifled by the lack of appropriate biochemical markers to characterize the changing physiological status of dormant vegetative or reproductive buds. Two sets of experiments were conducted in an attempt to identify changes in soluble protein profiles during endodormancy of peach and blueberry reproductive apices. Bud samples from the peach cultivars `La Festival' (low chilling requirement) and `La White' (moderate chilling requirement) were taken every 15 days in the orchard during December and January, extracted for soluble proteins, and analyzed by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Outshoots were forced at 25C in a growth chamber to determine the intensity of endodormancy. A further experiment utilized potted `Bluechip' and `Meader' (troth high chilling requirement) blueberry plants given varying periods of cold (4.5C) chamber treatment, followed by forcing at 25C in a growth chamber. Bud samples were taken following cold treatment for extraction and SDS-PAGE. The relationship of the resulting protein profiles to chilling unit accumulation and intensity of endodormancy will be discussed.

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