IN VITRO AND MACROPROPAGATION OF DYSSODIA PENTACHETA

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  • 1 Department of Horticultural Sciences, Texas A&M University, College Station, TX 77843-2133

Dyssodia pentacheta, a low growing perennial Texas wild flower with potential for use in low maitenance landscapes, was propagated in vitro and with cuttings under a mist system. Over 80% of both semihardwood terminal cutting from stock plants and in vitro grown nodal segments, dipped in 0, 3, 10, or 30 M-3 IBA, formed roots after 4 weeks under an intermittent mist system. A 300 M-3 IBA basal dip was lethal to the cuttings. Dyssodia produced significantly more shoots per nodal explant in vitro on semisolid (2 g l-1 Gelrite) WPM with 1-10 M-6 BA than combinations of BA and 0.5 M-6 NAA. Shoots were successfully subcultured and grown for two passes on semisolid growth regulator free medium. When maintaining Dyssodia in vitro on WPM, void of plant growth regulators, 1% sucrose promoted shoot growth and suppressed phenolic production better than 2% sucrose.

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