Commercial asparagus (Asparagusofficinalis L.) is currently planted from seed but there is a growing interest in the use of tissue culture clones. The worldwide occurrence of asparagus virus I (AV-I) and asparagus virus II (AV-II) in asparagus production areas has led to an investigation of the effect of these viruses singly and in combination on the propagation of asparagus via tissue culture. Bud explants from field-grown, virus-infected asparagus plants were cultured in-vitro to induce shoots and roots. Explants derived from singly or doubly-infected plants were slow to develop roots and often died in culture. The four virus groups were ranked for the explants' capacity to produce roots and shoots: virus-free > AV-II > AV-I> AV-I and AV-II. Plants derived from explants of AV-II-infected plants exhibited a mild weight reduction after three months in the greenhouse. Greater reductions were associated with AV-I and double infections when compared to healthy controls.