Separation of 2n Potato Pollen from a Heterogeneous Pollen Mixture by Velocity Sedimentation

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  • 1 Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456

A method is described for separating large and small pollen effectively from a heterogeneous mixture. This method potentially is applicable to separation of pollen grains of different ploidy levels, since “unreduced” 2n pollen is larger than normal pollen (n); it might then be used to increase the efficiency of a breeding program employing sexual polyploidization and to diminish crossing inefficiencies in interploid crosses.

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