Promotion of in Vitro Leaf Growth of Inner Scales Excised from Dormant Onion Bulbs

in HortScience
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  • 1 Department of Horticultural Sciences, Texas A&M University, College Station, TX 77843

Inner scales excised from dormant bulbs of the short-day `Texas Grano 1015Y' onion (Allium cepa L.) were cultured in vitro and leaf growth was examined. Light promoted leaf growth, but no differences in leaf growth were observed for media pH between 4 and 7. Leaf growth rate in darkness was highest at 24C, reduced at 15C, and greatly reduced at SC. Kinetin promoted leaf growth at 1, 10, and 100 μm. IAA was effective at 1 and 10 μM, but not at 0.1 and 100 μm. GA3 promoted growth at 0.1 μM. No inhibitory effects of ABA on leaf growth could be detected. Chemical names used: 1-H-indole-3-acetic acid (IAA), abscisic acid (ABA), gibberellic acid (GA3), 6-furfurylaminopurine (Kinetin).

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