Dormant bud explants taken from mature trees of Japanese persimmon cv. Hiratanenashi were established successfully on modified Murashige and Skoog's medium with nitrate reduced to half-strength [MS (½NO3)] or woody plant medium, both supplemented with 22.2 μM (5 mg°liter−1) BA. Shoot proliferation in subcultures also was best at 22.2 μM (5 mg°liter−1) BA in MS (½NO3) medium, but growth was of the rosette type. Shoot elongation, however, was stimulated the most in the same medium supplemented with 24.6 μM (5 mg°liter−1) 2iP instead of BA. Rooting of the proliferated shoots was enhanced by the treatment with IBA at 1.23 mM (250 mg°liter−1). Chemical names used: N-(phenylmethyl)-1H-purin-6-amine (BA), N-(3-methyl-2-butenyl)-1H-purin-6-amine (2iP), 1H-indole-3-butanoic acid (IBA).
Received for publication 30 Oct. 1985. The cost of publishing this paper was defrayed in part by the payment of page charges. Under postal regulations, this paper therefore must be hereby marked advertisement solely to indicate this fact.