Propagation of Desert Milkweed by Shoot Tip Culture

in HortScience
Authors:
Chi Won LeeDepartment of Plant Sciences, University of Arizona, Tucson, AZ 85721

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John C. ThomasDepartment of Plant Sciences, University of Arizona, Tucson, AZ 85721

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Abstract

Shoot tips and stem nodes of Asclepias erosa Torr., cultured on a modified (0.5 × major salts) Murashige and Skoog (MS) medium containing 0.54 µm (0.1 mg/liter) NAA and 44.4 µm (10 mg/liter) BA, produced multiple shoots in 5 weeks. Subcultures of the individual shoots on the same medium produced 5-12 new shoots 4 weeks later. Rooting of the resultant shoots was best accomplished by preculturing them for 48 hr on MS medium containing 246 or 492 µm (50 or 100 mg/liter) IB A prior to subculturing for 4 weeks on MS medium devoid of growth regulators. The rooted cultures were established successfully in soil. Chemical names used. NAA: 1-naphthaleneacetic acid. BA:N-(phenylmethyl)-lH-purin-6-amine. IBA: lH-indole-3-butanoic acid.

Contributor Notes

Received for publication 3 Aug. 1984. Journal article no. 3962 of the Univ. of Arizona Agr. Expt. Sta. The authors thank Janis Yeckes and Elaine Wagner for their technical assistance.The cost of publishing this paper was defrayed in part by the payment of page charges. Under postal regulations, this paper therefore must be hereby marked advertisement solely to indicate this fact.

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