Identification of Plum × Peach Hybrids by Isoenzyme Analysis

in HortScience
Authors:
D. E. ParfittDepartment of Pomology, University of California, Davis, CA 95616

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S. ArulsekarDepartment of Pomology, University of California, Davis, CA 95616

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D. W. RammingARS, USDA, Fresno, CA 93747

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Abstract

Two isoenzyme systems, glucose phosphate isomerase and phosphoglucomutase, were identified for use as starch gel electrophoretic markers of plum × peach (Prunus salicina × Prunus persica) interspecific hybrids. Two distinct regions of banding were associated with each enzyme system. Different unique banding patterns for each species were observed for plum and peach at 3 of 4 banding regions. Interspecific hybrid plants exhibited hybrid enzyme patterns with bands from both plum and peach in each region. Consequently, interspecific plum × peach hybrid genotypes may be distinguished from parental plum or peach genotypes. These enzyme systems may be used in breeding programs to identify plum × peach hybrid seedlings.

Contributor Notes

Assistant Pomologist.

Postgraduate Research Geneticist.

USDA Research Leader.

Received for publication 4 June 1984. This research was supported by USDA grant number 81-CRSR-2-0732. The authors acknowledge Anne Nooteboom for sample preparation, Ann von Kugelgen for typing, and Don Durzan and Gale McGranahan for manuscript review. The cost of publishing this paper was defrayed in part by the payment of page charges. Under postal regulations, this paper therefore must be hereby marked advertisement solely to indicate this fact.

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