A Citrus Embryo Assay to Screen Water-soluble Resins as Synthetic Seed Coats

in HortScience
Authors:
S. L. KittoDepartment of Horticulture, Purdue University, West Lafayette, IN 47907

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Jules JanickDepartment of Horticulture, Purdue University, West Lafayette, IN 47907

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Abstract

An assay using in vivo-produced embryos, with and without tegmen, of monoembryonic ‘Temple’ tangor [Citrus sinensis (L.) Osbeck x C. reticulata Blanco) and polyembryonic ‘Troyer’ citrange (C. sinensis x Poncirus trifoliata Raf.) was developed to screen compounds as synthetic seed coats for in vitro-produced, asexual embryos. Of 8 compounds evaluated, Polyox WSR-N 750 proved to be the most suitable as a synthetic seed coat based on its film-forming ability, its ability to redissolve in water, and its nondeleterious effects on citrus embryos.

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Received for publication 18 May 1984. Journal Paper No. 9867 of the Purdue Univ. Agricultural Experiment Station. We acknowledge the generosity of Charles Youtsey, Citrus Budwood Registration Bureau, Lake Alfred, Fla., who supplied seed of ‘Temple’ tangor and ‘Troyer’ citrange. This research was supported by a grant from the American Seed Trade Assn. The cost of publishing this paper was defrayed in part by the payment of page charges. Under postal regulations, this paper therefore must be hereby marked advertisement solely to indicate this fact.

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