In Vitro Propagation of Pecan Seedlings

in HortScience
Keith C. HansenDepartment of Horticultural Sciences, Texas A&M University, College Station, TX 77843

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Jaime E. LazartePlant Reproduction International, Inc., A Cornelius Company, P.O. Box 22626, Houston, TX 77027

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Single-node cuttings obtained from 2-month-old seedlings of pecan [Carya illinoinensis (Wang.) K. Koch] were induced to break buds and form multiple shoots in liquid, woody plant medium (WPM) and 2% glucose supplemented with 6-benzylamino purine (BA) at 3 mg/liter. In vitro-derived shoots soaked in 1-3 mg/liter indolebutyric acid (IBA) produced adventitious roots in vitro; when soaked for 8 days in 10 mg/liter IBA, they were rooted successfully in soil and acclimated to greenhouse conditions. Etiolation of stock plants did not improve shoot proliferation or rooting under in vitro culture.

Contributor Notes

Current address: Dept. of Pomology, University of California, Davis, CA 95616.

Received for publication 9 Dec. 1982. The authors gratefully acknowledge F.T. Davies, Jr., J. Benton Storey, and Stephen Newman of Texas A&M Univ. The cost of publishing this paper was defrayed in part by the payment of page charges. Under postal regulations, this paper therefore must be hereby marked advertisement solely to indicate this fact.

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