The storage life of ‘Clementine’ mandarin (Citrus reticulata Blanco) and Poncirus trifoliata (L.) Raf. pollen was effectively extended in an oxygen-free atmosphere. ‘Clementine’ pollen held at 4°C enabled satisfactory germination (20%) up to 7 weeks; a similar rate of germination was maintained after 20 weeks by use of N atmosphere. By combining deepfreeze temperatures (−18°) with either N or CO2 atmosphere for pollen storage, a germination rate of 13% was maintained after 57 weeks. With Poncirus, lower germination rates were obtained (7-9% after 57 weeks), but results paralleled those obtained with ‘Clementine’ mandarin pollen. Poncirus pollen, stored in an oxygen-free atmosphere at −18° for one year, maintained its fertilizing capacity.
Received for publication June 23, 1979. Contribution No. 179-E from Agricultural Research Organization, Israel.
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This constitutes part of a MSc Thesis of the first author submitted to the Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot.