In Vitro Propagation of Mammillaria elongata1

in HortScience
Authors:
J. L. JohnsonDepartment of Horticultural Sciences Texas A&M University, College Station, TX 77843

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E. R. EminoDepartment of Horticultural Sciences Texas A&M University, College Station, TX 77843

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Abstract

In vitro propagation of Mammillaria elongata DC plants was successful using tubercle explants grown on a medium based on Murashige and Skoog’s high salts supplemented with various auxins and cytokinins. Optimum callus proliferation occured in response to 2,4-dichlor-ophenoxyacetic acid (2,4-D) (2-10 mg/liter) with either kinetin or 6-(dimethylallylamino)-purine (2iP) (1-2 mg/liter). Root initiation was optimized with either napthaleneacetic acid (NAA) or indolebutyric acid (IBA) (60 mg/liter). Shoot initiation was optimized by addition of 2iP (10 mg/liter) and indoleacetic acid (IAA) (1 mg/liter). The auxin:cytokinin balance required for shoot initiation appears to be unique for each species of Mammillaria studied. Shoots developed in vitro of M. elongata were successfully transferred to greenhouse conditions, where they rooted and continued to grow.

Contributor Notes

Received for publication March 27. 1979. Texas Agricultural Experiment Station Journal Article 15256.

The cost of publishing this paper was defrayed in part by the payment of page charges. Under postal regulations, this paper must therefore be hereby marked advertisement solely to indicate this fact.

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