Carnation Propagation from Shoot Tips Cultured in Liquid Medium1

in HortScience
Authors:
E. D. EarleCornell University, Ithaca, New York

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R. W. LanghansCornell University, Ithaca, New York

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Abstract

Shoot tips of carnations (Dianthus caryophyllus L. cv. CSU White Pikes Peak) formed multiple shoots on agar nutrient medium containing 0.5 mg/liter kinetin and 0.1 mg/liter α-napthaleneacetic acid. Tissue with shoots was transferred to liquid medium on a culture wheel rotating 1 rpm. Many axillary shoots formed and eventually separated from the parent shoot. Tissue could be subcultured into fresh medium, stored at 4.5°, or rooted, potted and grown to flowering. All 175 plants flowered had normal white flowers with characteristic red flecks, indicating that the chimeral arrangement of the petal tissues had not been disturbed by the culture procedure.

Contributor Notes

Received for publication June 9, 1975.

Research Associate and Professor of Floriculture. We wish to thank Mrs. Barbara Stewart for her assistance.

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