Influence of Photoperiod and Culture Medium on the Speed of Asymbiotic Seed Germination and Seedling Development in Spathoglottis plicata

in HortScience

Spathoglottis plicata is an attractive, easy-to-grow, and floriferous terrestrial orchid that has become the most important horticulturally cultivated Spathoglottis species in Southeast Asia. The present research was conducted to study the asymbiotic seed germination and seedling development of this orchid under the influence of various photoperiod and medium treatments. Seeds from 28-day-old capsule were sown on five culture media, including half-strength Murashige and Skoog medium (1/2 MS), Orchid Seed Sowing Medium (OSSM), BM-1 Terrestrial Orchid Medium (BM-1), Vacin and Went Modified Orchid Medium (VW), and Knudson C Orchid Medium (KC), and incubated under 0/24-hour or 16/8-hour light/dark photoperiod. Seed germination occurred in all photoperiods and media tested but at different paces. Seeds subjected to total darkness germinated more rapidly in the first 3 weeks than those subjected to light. However, seed germination under light overtook or even exceeded seed germination under dark starting on the fourth week of culture. Seedlings grown on the OSSM and VW media showed the fastest development as they reached the advanced stage (Stage 6) within 11 weeks of culture. Seedlings on the BM-1 medium were the slowest to evolve, as they required more than 16 weeks’ time to complete all the developmental stages. Light-incubated advanced stage seedlings were subcultured on the same medium until leaves and roots were well developed and acclimatized in the greenhouse with 100% survival.

Contributor Notes

Corresponding author. E-mail: jyfang@mail.npust.edu.tw.

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    Overview of asymbiotic seed germination, seedling development, and seedling acclimatization in Spathoglottis plicata. (A) S. plicata flower. (B) Immature seed capsule. (C) Stage 1 seedlings with swollen embryos. (D) Stage 3 seedling with distinguishable protomeristem. (E) Stage 5 seedling with elongated first leaf. (F) Stage 6 seedling with two expanded leaves. (G) Seedlings before acclimatization. (H) Seedlings after acclimatization.

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    Percentages of germinated seeds of Spathoglottis plicata under the influence of different photoperiod and medium treatments over a 4-week culture period. Means (±se) with the same letter are not significantly different (α = 0.05).

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    Percentages of Spathoglottis plicata seedlings at different development stages on different media under light condition over a 16-week culture period. Means (±se) within each development stage with the same letter are not significantly different (α = 0.05).

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