Virulence Phenotypes on Chili Pepper for Phytophthora capsici Isolates from Michoacán, Mexico

in HortScience

Phytophthora blight of vegetables caused by Phytophthora capsici causes significant economic losses in production of Solanaceae and Cucurbitaceae crops in Mexico. The development of universal resistant chili pepper cultivars is challenging due to the diverse virulence phenotypes produced by P. capsici. The objective of the study was to characterize the diversity of phenotypic interactions for P. capsici isolates recovered from production fields in Michoacán, Mexico, to facilitate the development of resistant cultivars. Virulence phenotypes were characterized for 12 isolates of P. capsici using 26 Capsicum annuum New Mexico Recombinant Inbred Lines (NMRILs) in greenhouse conditions. Criollo de Morelos CM-334 and California Wonder were used as resistant and susceptible controls, respectively. Seedlings at the four to eight true leaf stage were inoculated with 10,000 zoospores per seedling and disease severity was evaluated at 20 days post-inoculation. Two of the P. capsici isolates did not infect any pepper host even though the isolate was less than a year old. The 10 virulent isolates were designated in 10 virulence phenotypes. The information generated by this study is of utmost importance for efforts of producing resistant cultivars specific for Michoacán producers.

Contributor Notes

The first author thanks the National Council for Science and Technology of Mexico (CONACYT) for his doctoral studies scholarship. The authors thank Paul W. Bosland of New Mexico State University for donating the NMRIL seeds set; José de Jesús Luna Ruiz of the Universidad Autónoma de Aguascalientes for donating the Criollo de Morelos CM334 seeds; and Daniela Pineda-Vaca and Erica Goss for skilled technical assistance.

This paper is a portion of a thesis presented by Alfredo Reyes-Tena, for the fulfillment of a degree requirement.

Corresponding author. E-mail: fpavia@umich.mx.

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    Plants with symptoms caused by Phytophthora capsici in the field: (A) wilted pepper plants in a field in Queréndaro, (B) pepper plants with root rot and stem necrosis in Queréndaro, and (C) wilted zucchini plant in Morelia.

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    Map of the sites where the Phytophthora capsici isolates were collected.

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    Disease symptoms exhibited in New Mexico Recombinant Inbred Lines (NMRIL) inoculated with Phytophthora capsici. Controls: (A) = CPV-279: CW (first three pots) and CM334 (second three pots), (B) = CPV-277: CW (first three pots) and CM334 (second three pots), (C) = CPV-259: CW (first three pots) and CM334 (second three pots). Phenotypic response in NMRIL-AA: (D) = CPV-279: CW (first three pots) and the susceptible NMRIL-AA (second three pots), (E) = CPV-277: CW (first three pots) and the susceptible NMRIL-AA, (F) CPV-259: CW (first three pots) and the resistant NMRIL-AA. Phenotypic response in NMRIL-AC: (G) = CPV-279: CW (first three pots) and the susceptible NMRIL-AC (second three pots), (H) = CPV-277: CW (first three pots) and the susceptible NMRIL-AC, (I) CPV-272: CW (first three pots) and the resistant NMRIL-AC.

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