Inexpensive plug-and-play temperature controllers have recently become available. These allow a chest freezer to be programmed easily to hold a desired set point across a range of biologically relevant temperatures. Installation can be completed in a few minutes using consumer-grade chest freezers. We used these temperature controllers to create five temperature-controlled chambers at 12, 14, 16, 18, and 20 °C. We demonstrated the use of these controlled-temperature chambers with two biologic assays: floral budbreak of peach [Prunus persica (L.) Batsch] stem cuttings and germination of sunflower (Helianthus annuus L.) seeds. We used the budbreak and germination rates at multiple temperatures to estimate base temperatures and thermal time requirements for development.
Funding for this project was provided from the Clemson University Creative Inquiry program and a grant to Douglas Bielenberg and Ksenija Gasic from the U.S. Department of Agriculture Specialty Crop Block Grant Program (agreement AM170100XXXXG034) through the South Carolina Department of Agriculture.
We thank Rosa Kome and Marcellus Washington for their invaluable assistance with data collection.
ZhebentyayevaT.N.FanS.ChandraA.BielenbergD.G.ReighardG.L.OkieW.R.AbbottA.G.2013Dissection of chilling requirement and bloom date QTLs in peach using a whole genome sequencing of sibling trees from an F2 mapping populationTree Genet. Genomes103551
Zhebentyayeva,T.N.Fan,S.Chandra,A.Bielenberg,D.G.Reighard,G.L.Okie,W.R.Abbott,A.G.2013Dissection of chilling requirement and bloom date QTLs in peach using a whole genome sequencing of sibling trees from an F2 mapping population103551)| false