Exploring Vegetative Propagation Techniques for the Threatened Pteroceltis tatarinowii Maxim Using Stem Cuttings

in HortScience

Pteroceltis tatarinowii Maxim, the only species of the genus Pteroceltis (family Ulmaceae), is an endemic rare tree species in China. This study was performed to explore vegetative propagation techniques for P. tatarinowii using stem cuttings. First, the effects of exogenous indole-3-butyric acid (IBA) and cutting positions on rooting performance were investigated to screen the appropriate exogenous auxin treatment and to determine the proper cuttings type. The results showed that the control cuttings pretreated with no exogenous IBA, irrespective of whether the stem cuttings were terminal, middle, or basal, rooted in a manner significantly inferior to that of cuttings pretreated with IBA. Their rooting percentage was less than 50%. Among the IBA-treated cuttings, the middle cuttings pretreated with 1000 mg·L−1 IBA rooted best, with the shortest number of days until rooting emergence (20 days), the highest rooting percentage (84.0%), the lowest mortality rate (4.0%), the greatest root number (average of 6.7 per cutting), and the longest roots per cutting (44.4 cm per cutting). Terminal cuttings pretreated with 1000 mg·L−1 IBA acquired satisfactory rooting traits and had the same shortest rooting duration (20 days) and the following parameters: rooting, 70.7%; mortality, 10.7%; average roots per cutting, 5.2; and longest root, 29.1 cm. To further determine the optimum cutting propagation time for this plant, a second experiment was performed and the cuttings were collected beginning in early June, when the growth of the current season was feasible for harvesting cuttings. Stem cuttings collected in late June and middle July had significantly higher rooting percentages (≥80%) compared with those collected in early June (66.7%). The other three rooting parameters were not significantly affected by the collection date. However, according to the overall rooting traits, the cuttings collected in both late June and middle July remarkably outperformed those collected in early June regarding the number of roots and the total root length per cutting. The initial nutrient reserves in the cuttings were also determined. A significant difference in the soluble carbohydrate level was found among collection times, but the nitrogen level in the cuttings was similar. The study revealed that stem cutting propagation of P. tatarinowii was achievable, and it was best achieved with cuttings collected from the terminal and middle positions of the branches of the current season from late June to middle July and treated with 1000 mg·L−1 IBA using the quick dip method.

Contributor Notes

This study was funded by the project of “collection, conservation, and evaluation of forest trees” (2010-6) sponsored by the Department of Science and Technology of Shandong Province, Shandong Provincial Agricultural Elite Varieties Project (2016LZGC038), and Forestry Science & Technology Innovation Project of Shandong Province (LYCX01-2018-03), China.

These authors contributed equally to this work.

Corresponding authors. E-mail: zangdk@sdau.edu.cn or guoxf@sdau.edu.cn.

Article Sections

Article Figures

  • View in gallery

    Growth of the branches of P. tatarinowii (A) and cuttings from different positions of the branches (B) during the current season.

  • View in gallery

    Morphological changes in P. tatarinowii cuttings and rooting variation when pretreated with different concentrations of IBA: (A) cuttings in the propagation shelters; (B) callus formation on day 5; (C) callus plump enlarged on day 10; (D) adventitious root emerged on day 20; (E) multiple adventitious roots formed on day 25; and (F) rooting response of middle cuttings varied with the IBA concentration on day 60.

  • View in gallery

    Effects of IBA concentrations and cutting positions on: (A) rooting percentage; (B) percent mortality; (C) mean root number per cutting; and (D) total root length. Notes: The same letters above the different columns indicate no significant difference at P ≤ 0.05. Error bars represent standard error.

Article References

  • AminahH.DickJ. McP.LeakeyR.R.B.GraceJ.SmithR.I.1995Effect of indole butyric acid (IBA) on stem cuttings of Shorea leprosulaFor. Ecol. Mgt.72199206

    • Search Google Scholar
    • Export Citation
  • AmriE.LyaruuH.V.M.NyomoraA.S.KanyekaZ.L.2010Vegetative propagation of African Blackwood (Dalbergia melanoxylon Guill. & Perr.): Effects of age of donor plant, IBA treatment and cutting position on rooting ability of stem cuttingsNew For.39183194

    • Search Google Scholar
    • Export Citation
  • AnG.C.ZhangC.J.WangL.AnF.2011Seeding technique of Pteroceltis tatarinowii using protective facilityPractical For. Technol.12224(in Chinese)

    • Search Google Scholar
    • Export Citation
  • CavinsJ.F.1980Nitrogen analysis of whole seedsJ. Amer. Oil Chem. Soc.57655656

  • DenaxaN.K.VemmosS.N.RoussosP.A.2012The role of endogenous carbohydrates and seasonal variation in rooting ability of cuttings of an easy and a hard to root olive cultivars (Olea europaea L.)Scientia Hort.143928

    • Search Google Scholar
    • Export Citation
  • DingT.HuangC.L.2012Research on the cutting propagation of Quercus rubraJ. of Anhui Agr. Univ.39507513(in Chinese with English abstr.).

    • Search Google Scholar
    • Export Citation
  • FangS.LiG.FuX.2004Biomass production and bark yield in the plantations of Pteroceltis tatarinowiiBiomass Bioenergy26319328

  • FuL.1991China plant red data book. Sci. Press. Beijing (in Chinese)

  • FuX.X.FangS.Z.WangH.W.HouC.Y.2001Studies on annual growth dynamics of one-year seedlings of Pteroceltis tatarinowiiJ. of Nanjing For. Univ.251114(Natural Sciences Edition)

    • Search Google Scholar
    • Export Citation
  • GuoS.J.LingH.Q.LiF.L.2004Physiological and biochemical basis of rooting of Pinus bungeana cuttingsJ. Beijing For. Univ.264347(in Chinese with English abstr.)

    • Search Google Scholar
    • Export Citation
  • GuoX.F.FuX.L.ZangD.K.MaY.2009Effect of auxin treatments, cuttings’ collection date and initial characteristics on Paeonia ‘Yang Fei Chu Yu’ cutting propagationScientia Hort.119177181

    • Search Google Scholar
    • Export Citation
  • HaileG.GebrehiwotK.LemenihM.BongersF.2011Time of collection and cutting sizes affect vegetative propagation of Boswellia papyrifera (Del.) Hochst through leafless branch cuttingsJ. Arid Environ.75873877

    • Search Google Scholar
    • Export Citation
  • HeS.G.YuT.Y.MaiL.F.2002Effects of IBA treatments on the root growth of hydroponic Aglaonema ‘Silver Queen’Acta Hort. Sinica.29288289(in Chinese with English abstr.)

    • Search Google Scholar
    • Export Citation
  • HowardB.H.1996Relations between shoot growth and rooting of cuttings in three contrasting species of ornamental shrubJ. Hort. Sci. Biotechnol.71591605

    • Search Google Scholar
    • Export Citation
  • HusenA.PalM.2007Effect of branch position and auxin treatment on clonal propagation of Tectona grandisNew For.34223233

  • JuliaA.C.JessicaD.L.2013Propagation of four underused native species from softwood cuttingsHortScience4810181020

  • KleinJ.D.CohenS.HebbeY.2000Seasonal variation in rooting ability of myrtle (Myrtus communis L.) cuttingsScientia Hort.837176

  • LiX.H.ZhangH.WangD.Y.ZhangL.ShaoJ.W.ZhangX.P.2013The genetic structure of endemic plant Pteroceltis tatarinowii by ISSR markersActa Ecol. Sin.3348924901(in Chinese with English abstr.)

    • Search Google Scholar
    • Export Citation
  • LiuC.C.LiuY.G.GuoK.FanD.Y.LiG.Q.ZhengY.R.YuL.F.YangR.2011Effect of drought on pigments, osmotic adjustment and antioxidant enzymes in six woody plant species in karst habitats of southwestern ChinaEnviron. Expt. Bot.71174183

    • Search Google Scholar
    • Export Citation
  • PhillipA.W.TimothyA.R.AdamJ.D.MarkP.LisaM.V.RyanM.RobertN.T.2014Propagation for the conservation of Pityopsis ruthii, an endangered species from the southeastern United StatesHortScience49194200

    • Search Google Scholar
    • Export Citation
  • SeverinoL.S.LimaR.L.S.LucenaA.M.A.FreireM.A.O.SampaioL.R.VerasR.P.MedeirosK.A.A.L.SofiattiV.ArrielN.H.C.2011Propagation by stem cuttings and root system structure of Jatropha curcasBiomass Bioenergy3531603166

    • Search Google Scholar
    • Export Citation
  • ShengM.D.2006Propagation technique by seeding of Pteroceltis tatarinowiiAn Hui Agr. Sci. Bull.12152177(in Chinese)

  • SinghB.YadavR.BhattB.P.2012Vegetative propagation of Dalbergia sissoo: Effect of growth regulators, length, position of shoot and type of cuttings on rooting potential in stem cuttingsFor. Stud. China14187192

    • Search Google Scholar
    • Export Citation
  • TworkoskiT.TakedaF.2007Rooting response of shoot cuttings from three peach growth habitsScientia Hort.11598100

  • ValerioC.YoussefR.EddoR.2010Collection time, cutting age, IBA and putrescine effects on root formation in Corylus avellana L. cuttingsScientia Hort.124189194

    • Search Google Scholar
    • Export Citation
  • WeiX.L.YuL.F.ZhuS.Q.XuX.Z.2007Effect of soil drying-wetting alternation on physiology and growth of Pteroceltis tatarinowii seedlingsScientia Silvae Sinicae.432328(in Chinese with English abstr)

    • Search Google Scholar
    • Export Citation
  • YangC.H.FangX.P.1996Seeding propagation of Pteroceltis tatarinowiiGuizhou For. Sci. and Technol.245356(in Chinese)

  • ZalesnyJ.R.S.HallR.B.BauerE.O.RiemenschneiderD.E.2003Shoot position affects root initiation and growth of dormant unrooted cuttings of PopulusSilvae Genet.525-6721724

    • Search Google Scholar
    • Export Citation
  • ZhouQ.2000Experimental guide of plant physiology. China Agr. Publ. House Beijing. 115–124 111–112 (in Chinese)

Article Information

Google Scholar

Related Content

Article Metrics

All Time Past Year Past 30 Days
Abstract Views 102 102 25
Full Text Views 32 32 12
PDF Downloads 20 20 5