Toxicity of Salicylic Acid in Cymbidium atropurpureo and Phalaenopsis Golden Peoker Cultivated In Vitro

in HortScience

Salicylic acid (SA) may induce toxicity in orchids depending on its concentration and the plant species, but there is no information about the effect of this substance on orchids cultivated in vitro. The objective of the present study was to evaluate the effects of different concentrations of SA on Cymbidium atropurpureo and Phalaenopsis Golden Peoker cultivated in vitro to verify the biological losses caused by the substance’s toxicity. The orchids Cymbidium atropurpureo and Phalaenopsis Golden Peoker were sown in vitro in a Murashige and Skoog (MS)-growth medium and transferred to a medium of the same type containing SA 90 days after sowing. The studied SA concentrations were 0, 50, 100, 200, 300, 400, 500, and 1000 µmol·L−1, and the plants were kept in this medium for 210 days. The treatments were distributed into a completely randomized design with four replications. Biometric variables of the seedlings and electrolyte leakage were evaluated 300 days after sowing. The results indicate that the addition of SA interfered with the in vitro growth and development of seedlings of Cymbidium atropurpureo and Phalaenopsis Golden Peoker, given that it caused all the examined variables to show reduced values and triggered electrolyte leakage, consequently inducing toxicity.

Contributor Notes

We express our gratitude to the Brazilian National Council for Scientific and Technological Development for providing the first author with a PhD scholarship.

Corresponding author. E-mail: orquidariomantovani@gmail.com.

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    Electrolyte leakage (A), number of leaves (B), leaf area (C), and shoot height (D) of Cymbidium atropurpureo and Phalaenopsis Golden Peoker as a function of exogenous salicylic acid concentrations after 300 d of in vitro cultivation. **P < 0.01.

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    Number of roots (A), length of the longest root (B), root dry weight (C), and shoot dry weight (D) of Cymbidium atropurpureo and Phalaenopsis Golden Peoker as a function of exogenous salicylic acid concentrations after 300 d of in vitro cultivation. **P < 0.01.

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    Seedlings of Cymbidium atropurpureo (A) and Phalaenopsis Golden Peoker (B) after treatment with increasing exogenous salicylic acid concentrations (0, 50, 100, 200, 300, 400, 500, and 1000 µmol·L−1) after 300 d of in vitro cultivation.

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