Rooting Ability of Hardwood Cuttings in Highbush Blueberry (Vaccinium corymbosum L.) Using Different Indole-butyric Acid Concentrations

in HortScience

Vegetative propagation by cuttings is a very popular method. However, blueberry propagation using cuttings is still a main factor limiting its expansion because its results can vary according to the blueberry cultivar and environmental factors. This study aimed to evaluate the rooting abilities of hardwood cuttings for six blueberry cultivars (O’Neal, Misty, Diana, Biloxi, Bluebeauty, and Coville) using three different exogenous indole-butyric acid (IBA) concentrations (1000, 2000, and 3000 ppm), and to determine if the cutting position (basal, central, apical) affects rooting performance. A control treatment (0 ppm IBA) was also performed. After 90 days of each treatment, rooting percentage, average root length, and average root number per cutting were assessed and used to calculate rooting index, which is a measure of rooting ability. The rooting percentages of hardwood cuttings differed largely among cultivars and were highest for ‘Bluebeauty’ (68.55%), followed by ‘Biloxi’ (68.01%). The rooting index values of these two cultivars (33.59 and 35.18, respectively) were significantly higher than those of the other four cultivars. The rooting response of blueberry hardwood cuttings to IBA concentrations was quadratic, and 1000 and 2000 ppm IBA were sufficient to express the maximum rooting percentage in most cultivars. The rooting abilities of basal, central, and apical cuttings were similar with treatments with high IBA concentrations. The effects of the cultivar, IBA concentration, and interaction between them on rooting percentage, average root length, and average root number were significant; however, the effects of the cutting position on the rooting percentage and average root length were not. This suggested that the rooting abilities of blueberry hardwood cuttings were significantly influenced by the cultivar and IBA concentration rather than by the cutting position.

Contributor Notes

We thank the Key Scientific and Technological Project of Agricultural System in Shanghai for funding (No. 17391900800).

These authors contributed equally to this work

Corresponding author. E-mail: zhangxueying@saas.sh.cn.

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    Rooting responses of hardwood cuttings from six blueberry cultivars (indicated at the top of each graphic) to different indole-butyric acid (IBA) concentrations (1000, 2000, or 3000 ppm). All data are the mean values of three replicates, and the curves were fitted by univariate regression using SPSS 18.0.

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    The rooting percentages of hardwood cuttings taken from the basal, central, and apical parts of the stem of six blueberry cultivars (indicated at the top of each graphic) using different indole-butyric acid (IBA) concentrations (1000, 2000, or 3000 ppm). All data are the mean values of three replicates, and the bar indicates the standard error. Different letters within the same treatment indicate significance at P ≤ 0.05 according to Duncan’s multiple range tests.

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    Average root numbers produced by hardwood cuttings taken from the basal, central, and apical parts of the stem of six blueberry cultivars (indicated at the top of each graphic) using different indole-butyric acid (IBA) concentrations (1000, 2000, or 3000 ppm). All data are the mean values of three replicates, and the bar indicates standard error. Different letters within the same treatment indicate significance at P ≤ 0.05 according to Duncan’s multiple range tests.

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    Rooting phenotype of the hardwood cuttings of blueberry cultivar ‘O’Neal’ treated with deionized water (control; A) and 1000 (B), 2000 (C), and 3000 (D) ppm IBA.

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    Rooting phenotype of the hardwood cuttings of blueberry cultivar ‘Misty’ treated with deionized water (control; A) and 1000 (B), 2000 (C), and 3000 (D) ppm IBA.

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    Rooting phenotype of the hardwood cuttings of blueberry cultivar ‘Diana’ treated with deionized water (control; A) and 1000 (B), 2000 (C), and 3000 (D) ppm IBA.

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    Rooting phenotype of the hardwood cuttings of blueberry cultivar ‘Coville’ treated with deionized water (control; A) and 1000 (B), 2000 (C), and 3000 (D) ppm IBA.

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    Rooting phenotype of the hardwood cuttings of blueberry cultivar ‘Biloxi’ treated with deionized water (control; A) and 1000 (B), 2000 (C), and 3000 (D) ppm IBA.

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    Rooting phenotype of the hardwood cuttings of blueberry cultivar ‘Bluebeauty’ treated with deionized water (control; A) and 1000 (B), 2000 (C), and 3000 (D) ppm IBA.

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