Characterization of Ethylene-mediated Curling of Japanese Radish (Raphanus sativus var. longipinnatus) Cotyledons

in HortScience

During the transport of vegetables, it is important to maintain quality. The cotyledons of Japanese radish (Raphanus sativus var. longipinnatus) sprouts curl during transport, lowering quality. It is known that ethylene causes the leaf curling of some true leaves by promoting cell growth on the adaxial side (epinasty); however, the mechanism of cotyledon curling is unknown. We investigated the effect of ethylene on cotyledon curling of Japanese radish sprouts. Curling was promoted by exogenous treatment with ethylene and repressed by treatment with 1-methylcyclopropene, an inhibitor of ethylene perception. Microscopic observation of ethylene-exposed curled cotyledons and normal cotyledons indicates that ethylene did not affect cell number but did inhibit transverse (lateral) cell growth on the abaxial side of the cotyledons, causing cotyledon curling through differential growth. Ethylene inhibition of cell growth on the abaxial side of leaves has not been reported before. We show a new mechanism responsible for curling.

Contributor Notes

We thank Masanori Tamaoki and Motoyuki Ashikari for providing 1-MCP used in this work. We also thank Ayami Takizawa for her technical assistance. This study was mainly supported by the University of Tsukuba GFEST program, the Global Science Campus from Japan Science and Technology Agency. Y.K. sincerely thanks the Ibaraki Prefectural Namiki Secondary School.N.N. is the corresponding author. E-mail: naka-320@nies.go.jp.
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