Powdery Mildew–Resistant Bottle Gourd Germplasm Lines: USVL351-PMR and USVL482-PMR

in HortScience

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USVL351-PMR (powdery mildew resistant) and USVL482-PMR are bottle gourd (Lagenaria siceraria) germplasm lines that exhibit high levels of resistance to the cucurbit powdery mildew (PM) pathogen (Podosphaera xanthii) compared with susceptible commercial bottle gourd rootstocks and other PIs. Powdery mildew is an important foliar disease affecting cucurbit crops grown in the United States and most parts of the world. It commonly occurs on seedlings, mature plant foliage, petioles, and stems. Resistance in these two germplasm lines is expressed as none to a few PM lesions, with sparse pathogen mycelia or conidia observed on young seedlings (including

Contributor Notes

This research was funded in part by a SCRI Vegetable Grafting grant award 2016-1498-08 and the SCRI CuCAP grant award 2015-51181-24285.

Mention of a trademark name or proprietary product does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture, Agricultural Research Service (USDA, ARS), nor does it imply exclusion of other products that may also be suitable.

We acknowledge the technical assistance of Danny Cook, Carrie Vanderspool, Steve Mayo, and numerous student workers over the years in conducting many of these experiments. Critical review of the manuscript by Drs. Livy Williams and Md. Shamimuzzaman is also appreciated.

Corresponding author. E-mail: shaker.kousik@ars.usda.gov.

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    Mature leaf from USVL351-PMR, powdery mildew–resistant bottle gourd (Lagenaria siceraria) germplasm line on left and the highly susceptible line USVL848-PMS on the right that is covered with white powdery mildew conidia.

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    Development of powdery mildew on cotyledons of the susceptible line USVL848-PMS (leaf on the left) and a commercial rootstock ‘Emphasis’ (leaf on right) compared with the two powdery mildew–resistant (PMR) germplasm lines USVL351-PMR and USVL482-PMR (in the middle) in greenhouse experiments.

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    True leaves from young seedling of USVL351-PMR, powdery mildew–resistant bottle gourd germplasm line on the left, and the highly susceptible line USVL848-PMS on the right showing white powdery mildew conidia.

  • View in gallery

    Hypocotyl of powdery mildew–resistant (PMR) bottle gourd germplasm line USVL351-PMR on the top and a highly susceptible line (USVL848-PMS; bottom), which is covered with abundant powdery mildew conidia.

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