Powdery Mildew–Resistant Bottle Gourd Germplasm Lines: USVL351-PMR and USVL482-PMR

in HortScience

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USVL351-PMR (powdery mildew resistant) and USVL482-PMR are bottle gourd (Lagenaria siceraria) germplasm lines that exhibit high levels of resistance to the cucurbit powdery mildew (PM) pathogen (Podosphaera xanthii) compared with susceptible commercial bottle gourd rootstocks and other PIs. Powdery mildew is an important foliar disease affecting cucurbit crops grown in the United States and most parts of the world. It commonly occurs on seedlings, mature plant foliage, petioles, and stems. Resistance in these two germplasm lines is expressed as none to a few PM lesions, with sparse pathogen mycelia or conidia observed on young seedlings (including

Contributor Notes

This research was funded in part by a SCRI Vegetable Grafting grant award 2016-1498-08 and the SCRI CuCAP grant award 2015-51181-24285.

Mention of a trademark name or proprietary product does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture, Agricultural Research Service (USDA, ARS), nor does it imply exclusion of other products that may also be suitable.

We acknowledge the technical assistance of Danny Cook, Carrie Vanderspool, Steve Mayo, and numerous student workers over the years in conducting many of these experiments. Critical review of the manuscript by Drs. Livy Williams and Md. Shamimuzzaman is also appreciated.

Corresponding author. E-mail: shaker.kousik@ars.usda.gov.

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    Mature leaf from USVL351-PMR, powdery mildew–resistant bottle gourd (Lagenaria siceraria) germplasm line on left and the highly susceptible line USVL848-PMS on the right that is covered with white powdery mildew conidia.

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    Development of powdery mildew on cotyledons of the susceptible line USVL848-PMS (leaf on the left) and a commercial rootstock ‘Emphasis’ (leaf on right) compared with the two powdery mildew–resistant (PMR) germplasm lines USVL351-PMR and USVL482-PMR (in the middle) in greenhouse experiments.

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    True leaves from young seedling of USVL351-PMR, powdery mildew–resistant bottle gourd germplasm line on the left, and the highly susceptible line USVL848-PMS on the right showing white powdery mildew conidia.

  • View in gallery

    Hypocotyl of powdery mildew–resistant (PMR) bottle gourd germplasm line USVL351-PMR on the top and a highly susceptible line (USVL848-PMS; bottom), which is covered with abundant powdery mildew conidia.

Article References

Ben-NaimY.CohenY.2015Inheritance of resistance to powdery mildew race 1W in watermelonPhytopathology10514461457

BrutonB.D.FishW.W.RobertsW.PophamW.2009The influence of rootstock selection on fruit quality attributes of watermelonOpen Food Sci. J.31534

CohenR.BurgerY.HorevC.KorenA.EdelsteinM.2007Introducing grafted cucurbits to modern agriculture. The Israeli experiencePlant Dis.91916923

CohenR.BurgerY.KatzirN.2004Monitoring physiological races of Podosphaera xanthii (syn. Sphaerotheca fuliginea), the causal agent of powdery mildew in cucurbits: Factors affecting race identification and the importance for research and commercePhytoparasitica32174183

CrosbyK.M.LeskovarD.JifonJ.L.MasabniJ.2015‘Pacal’ Orange Casaba: A multi-disease resistant, specialty melon cultivar from Texas A&M AgriLife ResearchHortScience5017231725

DavisA.R.Perkins-VeazieP.SakataY.Lopez-GalarzaS.MarotoJ.V.LeeS-G.HuhY-C.SunZ.MiguelA.KingS.R.CohenR.LeeJ-M.2008Cucurbit graftingCrit. Rev. Plant Sci.275074

DhillonN.P.S.SanguansilS.SrimatS.SchafleinterR.ManjunathB.AgarwalP.XiangQ.MasudM.A.T.MyintT.HanhN.T.CuongT.K.BalateroC.H.Salutam-BautistaV.PitratM.LebedaA.McCrieghtJ.D.2018Cucurbit powdery mildew-resistant bitter gourd breeding lines reveal four races of Podosphaera xanthii in AsiaHortScience53337

HultengrenR.L.WyattL.MazourekM.2016A suite of high-quality butternut squashHortScience5114351437

JahnM.MungerH.M.McCreightJ.D.2002Breeding cucurbit crops for powdery mildew resistance p. 239–248. In: R.R. Belanger W.R. Bushnell A.J. Dik and T.L.W. Carver (eds.). Chapter 15. The powdery mildews. A comprehensive treatise. APS Press St. Paul MN

KeinathA.P.2015Efficacy of fungicides against powdery mildew on watermelon caused by Podosphaera xanthiiCrop Protection757076

KeinathA.P.DuBoseV.B.2004Evaluation of fungicides for prevention and management of powdery mildew on watermelonCrop Protection233542

KeinathA.P.DuBoseV.B.2012Controlling powdery mildew on cucurbit rootstock seedlings in the greenhouse with fungicides and biofungicidesCrop Protection42338344

KeinathA.P.HassellR.2014Control of Fusarium wilt of watermelon by grafting onto bottle gourd or interspecific hybrid squash despite colonization of rootstocks by FusariumPlant Dis.98255266

KeinathA.P.RennbergerG.2017Powdery mildew on watermelon. Clemson Coop. Ext. Publ. Fact Sheet. Hort. 02. 12 Sept. 2017. <https://www.clemson.edu/extension/publications/files/horticulture/HOR02%20Powdery%20Mildew%20on%20Watermelon.pdf>

KingS.R.DavisA.R.LiuW.LeviA.2008Grafting for disease resistanceHortScience4316731676

KingS.R.DavisA.R.ZhangX.P.CrosbyK.2010Genetics, breeding and selection of rootstocks for Solanaceae and CucurbitaceaeScientia Hort.127106111

KousikC.S.DonahooR.S.WebsterC.G.TurechekW.W.AdkinsS.T.RobertsP.D.2011Outbreak of cucurbit powdery mildew on watermelon fruit caused by Podosphaera xanthii in southwest FloridaPlant Dis.951586

KousikC.S.HassellR.L.2009Evaluation of commercial watermelon rootstocks for tolerance to crown rot and powdery mildew, 2007Plant Dis. Mgt. Rpt.3V144

KousikC.S.IkerdJ.L.2013Confirming resistance in bottle gourd germplasm by quantifying powdery mildew conidia using a cellometerPhytopathology103S2.75(abstr.)

KousikC.S.IkerdJ.L.2016Evaluation of watermelon varieties for tolerance to powdery mildew and phytophthora fruit rot, 2014Plant Dis. Mgt. Rpt.10V082

KousikC.S.LeviA.LingK.S.WechterW.P.2008Potential sources of resistance to cucurbit powdery mildew in U.S. plant introductions of bottle gourdHortScience4313591364

KousikC.S.MandalM.K.HassellR.2018Powdery mildew resistant rootstocks that impart tolerance to grafted susceptible watermelon scion seedlingsPlant Disease10212901298

LaPlantK.E.WyattL.E.MoriartyG.Fink-BrodnickiM.JahnM.MazourekM.2016Powdery mildew–resistant pumpkin inbred linesHortScience5112971300

LeeJ.M.1994Cultivation of grafted vegetables. 1. Current status, grafting methods and benefitsHortScience29235239

MaddenL.V.HughesG.Van den BoschF.2007The study of plant disease epidemics. APS Press St. Paul MN

McGrathM.T.2010Managing cucurbit powdery mildew organically. Dec. 2017. <http://www.extension.org/pages/30604/managing-cucurbit-powdery-mildew-organically>

McGrathM.T.2017Powdery mildew p. 62–64. In: A.P. Keinath W.M. Wintermantel and T.A. Zitter (eds.). Compendium of cucurbit diseases and pests. 2nd ed. APS Press St. Paul MN

YetisirH.KurtS.SariN.TokF.M.2007Rootstock potential of Turkish Lagenaria siceraria germplasm for watermelon: Plant growth, graft compatibility, and resistance to FusariumTurk. J. Agr. For.31381388

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