Watermelon Germplasm Lines USVL608-PMR, USVL255-PMR, USVL313-PMR, and USVL585-PMR with Broad Resistance to Powdery Mildew

in HortScience

If the inline PDF is not rendering correctly, you can download the PDF file here.

Contributor Notes

Financial support was provided in part by USDA NIFA SCRI CuCAP grant award 2015-51181-24285.

We acknowledge the technical assistance of Jerry Johnson, Stephen Mayo, Carrie Vanderspool, and the numerous student workers over the years in conducting the greenhouse and field experiments. We appreciate the critical review of this manuscript by Phillip Wadl and Daniel Hasegawa before submission.

The use of trade, firm, or corporation names in this publication is for the convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agriculture Research Service of any product or service to the exclusion of others that may also be suitable.

Corresponding author. E-mail: shaker.kousik@ars.usda.gov.

Article Sections

Article Figures

  • View in gallery

    Powdery mildew–resistant (PMR) watermelon (Citrullus lanatus var. lanatus) germplasm line USVL608-PMR (on left) compared with susceptible line USVL677-PMS (right) in a greenhouse evaluation. The seedlings were inoculated with a local isolate (B108ML) of Podosphaera xanthii collected in Charleston, SC. Notice abundant P. xanthii conidia on USVL677-PMS compared with none on USVL608-PMR.

  • View in gallery

    Comparison of cotyledons of powdery mildew–resistant (PMR) watermelon (Citrullus lanatus var. lanatus) germplasm lines with susceptible line USVL677-PMS in a greenhouse trial. Seedlings were inoculated with a local isolate of Podosphaera xanthii (B108ML). Notice abundant P. xanthii conidia on cotyledon of USVL677-PMS (A) compared with the four PMR lines (BE). (A) USVL677-PMS, (B) USVL608-PMR, (C) USVL255-PMR, (D) USVL313-PMR, and (E) USVL585-PMR.

  • View in gallery

    Maintenance of isolates of the causal agent of powdery mildew, Podosphaera xanthii on cotyledons of “Early Prolific Straightneck” (EPSN) squash (Cucurbita pepo) in a petri dish with water agar covered with a blue blotter paper. Notice abundant development of P. xanthii conidia on cotyledon of EPSN. Each isolate used in the study was maintained similarly in individual petri dishes.

  • View in gallery

    Reaction of powdery mildew–resistant (PMR) lines to Podosphaera xanthii isolates, panel 1 from California (CADWM) and Panel 2 from Florida (GC1). All the true leaves of the four USVL-PMR lines were resistant to isolates from different states in petri dish assays. Notice abundant P. xanthii conidia on the true leaves of USVL677-PMS (A) compared with the four PMR lines (BE). (A) USVL677-PMS, (B) USVL608-PMR, (C) USVL585-PMR, (D) USVL313-PMR, and (E) USVL255-PMR.

  • View in gallery

    Fruit characteristics of USVL developed powdery mildew–resistant (PMR) watermelon germplasm lines USVL608-PMR, USVL255-PMR, USVL313-PMR, and USVL585-PMR. These four PMR germplasm lines can be easily crossed with other watermelon lines to develop populations for breeding superior quality watermelon varieties with resistance.

Article References

  • BardinM.SulimanM.E.Sage-PalloixsA-M.MohamedY.F.NicotP.C.2007Inoculum production and long term conservation methods for cucurbits and tomato powdery mildewsMycol. Res.111740747

    • Search Google Scholar
    • Export Citation
  • Ben-NaimY.CohenY.2015Inheritance of resistance to powdery mildew race 1W in watermelonPhytopathology10514461457

  • CohenR.BurgerY.KatzirN.2004Monitoring physiological races of Podosphaera xanthii (syn. Sphaerotheca fuliginea), the causal agent of powdery mildew in cucurbits: Factors affecting race identification and the importance for research and commercePhytoparasitica32174183

    • Search Google Scholar
    • Export Citation
  • DavisA.R.BrutonB.D.PairS.D.ThomasC.E.2001Powdery mildew: An emerging disease of watermelon in the United StatesCucurbit Genet. Coop.244248

    • Search Google Scholar
    • Export Citation
  • DavisA.R.LeviA.TettehA.WehnerT.C.RussoV.PitratM.2007Evaluation of watermelon and related species for resistance to race 1W powdery mildewJ. Amer. Soc. Hort. Sci.132790795

    • Search Google Scholar
    • Export Citation
  • DhillonN.P.S.SanguansilS.SrimatS.SchafleinterR.ManjunathB.AgarwalP.XiangQ.MasudM.A.T.MyintT.HanhN.T.CuongT.K.BalateroC.H.Salutam-BautistaV.PitratM.LebedaA.McCrieghtJ.D.2018Cucurbit powdery mildew-resistant bitter gourd breeding lines reveal four races of Podosphaera xanthii in AsiaHortScience53337

    • Search Google Scholar
    • Export Citation
  • GunerN.StrangeB.E.WehnerT.C.Pesic-VanEsbroeckZ.2002Methods for screening watermelon for resistance to Papaya ringspot virus type-WScientia Hort.94297307

    • Search Google Scholar
    • Export Citation
  • JahnM.MungerH.M.McCreightJ.D.2002Breeding cucurbit crops for powdery mildew resistance p. 239–248. In: R.R. Belanger W.R. Bushnell A.J. Dik and T.L.W. Carver (eds.). A comprehensive treatise Chapter 15: The powdery mildews. APS Press St. Paul MN

  • KeinathA.P.2015Efficacy of fungicides against powdery mildew on watermelon caused by Podosphaera xanthiiCrop Protection757076

  • KeinathA.P.DuBoseV.B.2004Evaluation of fungicides for prevention and management of powdery mildew on watermelonCrop Protection233542

  • KeinathA.P.RennbergerG.2017Powdery mildew on watermelon. Clemson Coop. Ext. Publ. Fact Sheet. Hort. 02. 12 Sept. 2017. <https://www.clemson.edu/extension/publications/files/horticulture/HOR02%20Powdery%20Mildew%20on%20Watermelon.pdf>

  • KousikC.S.AdkinsS.TurechekW.W.RobertsP.D.2009Sources of resistance in U.S. plant introductions (PI) to watermelon vine decline caused by Squash vein yellowing virusHortScience44256262

    • Search Google Scholar
    • Export Citation
  • KousikC.S.BruscaJ.TurechekW.W.2016Diseases and disease management strategies take top research priority in the watermelon research and development group members survey (2014–15)Plant Health Prog.175358

    • Search Google Scholar
    • Export Citation
  • KousikC.S.DonahooR.S.WebsterC.G.TurechekW.W.AdkinsS.T.RobertsP.D.2011Outbreak of cucurbit powdery mildew on watermelon fruit caused by Podosphaera xanthii in southwest FloridaPlant Dis.951586

    • Search Google Scholar
    • Export Citation
  • KousikC.S.IkerdJ.L.2014Evidence for cucurbit powdery mildew pathogen races based on watermelon differentials p. 32–34. In: M. Havey Y. Weng B. Day and R. Grumet (eds.). Proceedings of cucurbitaceae 2014. Amer. Soc. Hort. Sci. Alexandria VA

  • KousikC.S.IkerdJ.L.2016Evaluation of watermelon varieties for tolerance to powdery mildew and Phytophthora fruit rot, 2014Plant Dis. Mgt. Rpt.10V082

    • Search Google Scholar
    • Export Citation
  • KousikC.S.MandalM.K.HassellR.2018Powdery mildew resistant rootstocks that impart tolerance to grafted susceptible watermelon scion seedlings. Plant Disease. First Look paper. <https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-09-17-1384-RE>

  • LebedaA.KristkovaE.SedlakovaB.2011Gaps and perspectives of pathotype and race determination in Golovinomyces cichoracearum and Podosphaera xanthiiMycoscience52159164

    • Search Google Scholar
    • Export Citation
  • LebedaA.KristkovaE.SedlakovaB.McCreightJ.D.CoffeyM.D.2016Cucurbit powdery mildews: Methodology for objective determination and denomination of racesEur. J. Plant Pathol.144399410

    • Search Google Scholar
    • Export Citation
  • MaddenL.V.HughesG.Van den BoschF.2007The study of plant disease epidemics. Amer. Phytopathol. Soc. Press St. Paul MN

  • McCreightJ.D.2006Melon-powdery mildew interactions reveal variation in melon cultigens and Podosphaera xanthii races 1 and 2J. Amer. Soc. Hort. Sci.1315965

    • Search Google Scholar
    • Export Citation
  • McGrathM.T.2010Managing cucurbit powdery mildew organically. 7 Jan. 2018. <http://www.extension.org/pages/30604/managing-cucurbit-powdery-mildew-organically>

  • McGrathM.T.2017Powdery mildew p. 62–64. In: A.P. Keinath W.M. Wintermantel and T.A. Zitter (eds.). Compendium of cucurbit diseases and pests. 2nd ed. APS Press St. Paul MN

  • MercierJ.MuscaraM.J.DavisA.R.2014First report of Podosphaera xanthii race 1W causing powdery mildew of watermelon in CaliforniaPlant Dis.98158

    • Search Google Scholar
    • Export Citation
  • PitratM.DogimontC.BardinM.1998Resistance to fungal diseases of foliage in melon p. 167–173. In: J.D. McCreight (ed.). Cucurbitaceae ’98. ASHS Press Alexandria VA

  • StrangeB.E.GunerN.Pesic-VanEsbroeckZ.WehnerT.C.2002Screening the watermelon germplasm collection for resistance to Papaya ringspot virus type-WCrop Sci.4213241330

    • Search Google Scholar
    • Export Citation
  • TettehA.Y.WehnerT.C.DavisA.R.2010Identifying resistance to powdery mildew race 2W in the USDA-ARS watermelon germplasm collectionCrop Sci.50933939

    • Search Google Scholar
    • Export Citation
  • ThomasC.E.LeviA.CanigliaE.2005Evaluation of U.S. plant introductions of watermelon for resistance to powdery mildewHortScience40154156

    • Search Google Scholar
    • Export Citation
  • ZhangH.GuoS.GongG.RenY.DavisA.R.XuY.2011Sources of resistance to race 2WF powdery mildew in US watermelon plant introductionsHortScience4613491352

    • Search Google Scholar
    • Export Citation

Article Information

Google Scholar

Related Content

Article Metrics

All Time Past Year Past 30 Days
Abstract Views 39 39 5
Full Text Views 26 26 9
PDF Downloads 10 10 0