Effects of Exogenous 6-Benzyladenine on Dwarfing, Shoot Branching, and Yield of Tea Plant (Camellia sinensis)

in HortScience

6-Benzyladenine (6-BA) is a safe and efficient cytokinin. The adult tea plants of the cv. Longjing 43 were used in this study. The foliar portion of tea bushes were sprayed with different concentrations (50, 100, 200, or 400 mg·L−1) of 6-BA after heavy pruning, when three to four leaves grew out in late May. The effects of 6-BA application on the growth of the new shoots and lateral branches were quantified. After 5 months, treatments with 50, 100, 200, or 400 mg·L−1 6-BA suppressed plant height by 11.0%, 18.0%, 21.0%, or 22.0%, respectively; 6-BA at 100, 200, or 400 mg·L−1 decreased the number of lateral branches by 20.0%, 23.0%, or 18.0%, respectively. Meanwhile, treatments with 50, 200, or 400 mg·L−1 6-BA increased the length of lateral branches by 38.0%, 79.0%, or 81.0% respectively; 200 mg·L−1 6-BA increased the diameter of lateral branches by 8.0%. In addition, after 2 months, 50 or 200 mg·L−1 6-BA did not significantly affect the growth of functional leaves, 50, 100, or 200 mg·L−1 6-BA did not significantly affect photosynthetic rate (Pn) as compared with the control. Furthermore, 200 or 400 mg·L−1 6-BA significantly increased spring tea yield by 28.9% or 13.3%, respectively as compared with the control. In conclusion, 6-BA at the four concentrations promoted dwarfing and the formation of productive lateral branches and increased the spring yield, and 200 mg·L−1 6-BA exerted the best comprehensive effect.

Contributor Notes

This study was funded by the Innovation Project of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-2015-TRICAAS-08) and also by the Key Project of International Science & Technology Cooperation, National Key Research and Development Programme of China (2017YFE0107500).

Corresponding author. E-mail: hanwy@tricaas.com.

  • AngerR.H.M.PrasadP.C.LaudeH.M.1973Effects of kinetin on tiller bud elongation in wheat (Triticum aestivum L.)Ann. Bot.37565571

  • BrennerW.G.SchmüllingT.2012Transcript profiling of cytokinin action in Arabidopsis roots and shoots discovers largely similar but also organ-specific responsesBMC Plant Biol.12112

    • Search Google Scholar
    • Export Citation
  • ChatfieldS.P.StirnbergP.FordeB.G.LeyserO.2000The hormonal regulation of axillary bud growth in ArabidopsisPlant J.24159169

  • ChenX.J.XiaX.J.GuoX.ZhouY.H.ShiK.ZhouJ.YuJ.Q.2016Apoplastic H2O2 plays a critical role in axillary bud outgrowth by altering auxin and cytokinin homeostasis in tomato plantsNew Phytol.21112661278

    • Search Google Scholar
    • Export Citation
  • DierckR.KeyserE.D.RiekJ.D.DhoogheE.HuylenbroeckJ.V.PrinsenE.StraetenD.V.D.2016Change in auxin and cytokinin levels coincides with altered expression of branching genes during axillary bud outgrowth in ChrysanthemumPLoS One118e0161732

    • Search Google Scholar
    • Export Citation
  • DunE.A.GermainA.S.RameauC.BeveridgeC.A.2012Antagonistic action of strigolactone and cytokinin in bud outgrowth controlPlant Physiol.158487498

    • Search Google Scholar
    • Export Citation
  • ElfvingD.C.VisserD.B.2006Timing cyclanilide and cytokinin applications in the nursery to obtain desired lateral branch height in apple and sweet cherry treesHortScience4112381242

    • Search Google Scholar
    • Export Citation
  • EmeryR.J.N.LongneckerN.E.AtkinsC.A.1998Branch development in Lupinus angustifolius L. II. Relationship with endogenous ABA, IAA and cytokinins in axillary and main stem budsJ. Expt. Bot.49320555562

    • Search Google Scholar
    • Export Citation
  • HitoshiS.KentaroT.NaoyaH.2006Interactions between nitrogen and cytokinin in the regulation of metabolism and developmentTrends Plant Sci.11440448

    • Search Google Scholar
    • Export Citation
  • HondaC.KusabaS.NishijimaT.MoriguchiT.2011Transformation of kiwifruit using the ipt gene alters tree architectureJ. Plant Biotech.1074553

    • Search Google Scholar
    • Export Citation
  • HuangW.D.HanZ.H.LiuS.XuX.F.LiB.1999Effects of point-daub with 6-BA ointment on bud breaking, shoot growth, and the shaping of young apple trees (in Chinese)Rev. China Agr. Sci. Technol.27275

    • Search Google Scholar
    • Export Citation
  • KagiraC.N.1975Stimulation of branching in young tea plantsActa Hort.49137146

  • KakimotoT.2003Perception and signal transduction of cytokininsAnnu. Rev. Plant Biol.54605627

  • KenderW.J.CarpenterS.1972Stimulation of lateral bud growth of apple trees by 6-benzylamino purineJ. Amer. Soc. Hort. Sci.97377380

  • LiX.AhammedG.J.LiZ.X.ZhangL.WeiJ.P.ShenC.YanP.ZhangL.P.HanW.Y.2016Brassinosteroids improve quality of summer tea (Camellia sinensis L.) by balancing biosynthesis of polyphenols and amino acidsFront. Plant Sci.71304

    • Search Google Scholar
    • Export Citation
  • LiX.G.1990The effects of cytokinin spraying on the tea plants (in Chinese). Chinese tea 2:33

  • LiX.Y.QianQ.FuZ.M.WangY.H.XiongG.S.ZengD.L.WangX.Q.LiuX.F.TengS.HiroshiF.YuanM.LuoD.HanB.LiJ.Y.2003Control of tillering in riceNature422618621

    • Search Google Scholar
    • Export Citation
  • LiangY.LuJ.ShangS.1997Effect of gibberellins on chemical composition and quality of tea (Camellia sinensis L)J. Sci. Food Agr.72411414

    • Search Google Scholar
    • Export Citation
  • LiuY.WangQ.S.DingY.F.LiuZ.H.LiG.H.WangS.H.2009Effect of nitrogen and 6-BA on development of tillering bud and its physiological mechanism (in Chinese)Acta Agron. Sinica351018931899

    • Search Google Scholar
    • Export Citation
  • MauduM.E.MudauF.N.MarigaI.K.2011The effect of gibberellins on sprouting of cuttings and quality of bush tea (Athrixia phylicoides DC)Afr. J. Biotechnol.104487418745

    • Search Google Scholar
    • Export Citation
  • McSteenP.LeyserO.2005Shoot branchingAnnu. Rev. Plant Biol.56353374

  • MengY.MaS.ShaoJ.SunJ.MaB.WangH.2012Effects of spraying 6-BA on axillary bud growth and the dynamic changes of endogenous hormones in ‘Tianhong 2’ Fuji nursery apple trees (in Chinese)Acta Hort. Sinica395837844

    • Search Google Scholar
    • Export Citation
  • MuhrM.PrüferN.PaulatM.TeichmannT.2016Knockdown of strigolactone biosynthesis genes in Populus affects BRANCHED1 expression and shoot architectureNew Phytol.212613626

    • Search Google Scholar
    • Export Citation
  • NiJ.GaoC.ChenM.S.PaB.Z.YeK.XuZ.F.2015Gibberellin promotes shoot branching in the perennial woody plant Jatropha curcasPlant Cell Physiol.56816551666

    • Search Google Scholar
    • Export Citation
  • OnoT.TamaiH.MaejimaT.UsudaA.KoikeH.OharaH.2005Effects of repeated benzyladenine spraying on branch development of apple nursery trees on M.9 rootstocksHort. Res.42165170

    • Search Google Scholar
    • Export Citation
  • RoversiA.1971The branching property of morphactin on Prunus avium seedlingsExperientia271012331234

  • SachsT.ThimannK.V.1967The role of auxins and cytokinins in the release of buds from dominanceAmer. J. Bot.54136144

  • SakakibaraH.TakeiK.HiroseN.2006Interactions between nitrogen and cytokinin in the regulation of metabolism and developmentTrends Plant Sci.11440448

    • Search Google Scholar
    • Export Citation
  • Shimizu-SatoS.TanakaM.MoriH.2009Auxin–cytokinin interactions in the control of shoot branchingPlant Mol. Biol.69429435

  • SprentJ.I.1967The effects of benzyladenine on the growth and development of peasPlanta7811724

  • TounektiT.JoubertE.HernándezI.Munné-BoschS.2013Improving the polyphenol content of teaCrit. Rev. Plant Sci.32192215

  • WernerT.MotykaV.LaucouV.SmetsR.OnckelenH.V.SchmüllingT.2003Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activityPlant Cell1525322550

    • Search Google Scholar
    • Export Citation
  • WernerT.MotykaV.StrnadM.SchmullingT.2001Regulation of plant growth by cytokininProc. Natl. Acad. Sci. U.S.A.981048710492

  • WuZ.J.LiX.H.LiuZ.W.LiH.WangY.X.ZhuangJ.2015Transcriptome-based discovery of AP2/ERF transcription factors related to temperature stress in tea plant (Camellia sinensis)Funct. Integr. Genomics15741752

    • Search Google Scholar
    • Export Citation
  • XuW.SongQ.LiD.WanX.2012Discrimination of the production season of Chinese green tea by chemical analysis in combination with supervised pattern recognitionJ. Agr. Food Chem.6070647070

    • Search Google Scholar
    • Export Citation
  • YaoG.WuX.1990Formation and regulation of the “optimum type” of tea plant structure (in Chinese)Scientia Agr. Sinica2366268

  • YuJ.DongL.L.XiL.ZhaoR.Y.MaN.ZhaoL.J.2012Isolation and characterization of cytokinin synthase gene DgIPT3 in Chrysanthemum ‘Jinba’ (in Chinese)Acta Hort. Sinica394721728

    • Search Google Scholar
    • Export Citation
  • YueC.ZengJ.ZhangZ.WangX.CaoH.2012Research progress in the phytohormone of tea plant (Camellia sinensis) (in Chinese)J. Tea Sci.325382392

    • Search Google Scholar
    • Export Citation
  • ZengX.F.ZhaoD.G.2016Expression of IPT in Asakura-sanshoo (Zanthoxylum piperitum (L.) DC. f. inerme Makino) alters tree architecture, delays leaf senescence, and changes leaf essential oil compositionPlant Mol. Biol. Rpt.34649658

    • Search Google Scholar
    • Export Citation
All Time Past Year Past 30 Days
Abstract Views 351 351 36
Full Text Views 55 55 1
PDF Downloads 10 10 0