Mitigating Effect of Glycinebetaine Pretreatment on Drought Stress Responses of Creeping Bentgrass

in HortScience

Turfgrass performance under drought stress is impeded by plant water deficit and oxidative damage, which might be improved by the external application of osmoprotectants. Creeping bentgrass (Agrostis stolonifera L.) is a valuable species for low-cut golf surfaces as a result of its high density and fine texture. However, weak tolerance to drought stress is a primary shortcoming. In this study, the effect of exogenous glycinebetaine (GB) pretreatment on mitigating the damage from drought stress in creeping bentgrass cultivar ‘T-1’ was evaluated. Pieces of creeping bentgrass sod were subjected to four treatments: 1) well-watered control, 2) well watered and sprayed with 100 mm GB, 3) drought stress, and 4) drought stress and sprayed with 100 mm GB. Drought stress resulted in a remarkable decrease in turf quality (TQ), relative water content (RWC), and chlorophyll content, with significant increases in superoxide anion content (O2), malondialdehyde (MDA) content, superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activity. In contrast, pretreatment with 100 mm GB decreased the O2 and MDA content in water-stressed plants, and increased turf quality, chlorophyll content, SOD, CAT, and POD activity. Meanwhile, the expression level of the psbA, SAMS4, CMO, and ACS1 genes in leaf samples collected during the drought-stress stage was elevated in GB pretreatment. Notably, SAMS4 gene expression in GB pretreatment was significantly greater than in the untreated GB groups subjected to water stress. These results suggested that GB could mitigate the adverse effect of water stress on creeping bentgrass. The amelioration related strongly to the maintenance of the antioxidant enzyme system, accumulated endogenous compatible metabolites, and the elevation of gene expression levels. These findings lead us to conclude that GB pretreatment could be used as an ameliorative agent for creeping bentgrass against the deleterious effects of water stress.

Contributor Notes

This study was supported by the National Natural Science Foundation of China (no. 31302016) and the Science and Technology Program of Shenzhen (JCYJ20160331151245672 and JSGG20160229155434792).

Corresponding author. E-mail: yinsx369@bjfu.edu.cn.

  • ApelbaumA.YangS.F.1981Biosynthesis of stress ethylene induced by water deficitPlant Physiol.68594596

  • ArnonD.I.1949Copper enzymes in isolated chloroplasts: Polyphenoloxidase in Beta vulgarisPlant Physiol.241

  • AshrafM.2010Inducing drought tolerance in plants: Recent advancesBiotechnol. Adv.28169183

  • AshrafM.FooladM.2007Roles of glycine betaine and proline in improving plant abiotic stress resistanceEnviron. Expt. Bot.59206216

  • BohnertH.J.JensenR.G.1996Strategies for engineering water-stress tolerance in plantsTrends Biotechnol.148997

  • BurgessP.HuangB.2014Effects of sequential application of plant growth regulators and osmoregulants on drought tolerance of creeping bentgrass (Agrostis stolonifera)Crop Sci.54837844

    • Search Google Scholar
    • Export Citation
  • BurnetM.LafontaineP.J.HansonA.D.1995Assay, purification, and partial characterization of choline monooxygenase from spinachPlant Physiol.108581588

    • Search Google Scholar
    • Export Citation
  • ChanceB.MaehlyA.1955Assay of catalases and peroxidases p. 764–775. Methods in Enzymology. Academic Press

  • DacostaM.HuangB.2007Changes in antioxidant enzyme activities and lipid peroxidation for bentgrass species in response to drought stressJ. Amer. Soc. Hort. Sci.132319326

    • Search Google Scholar
    • Export Citation
  • DawoodM.TaieH.NassarR.AbdelhamidM.SchmidhalterU.2014The changes induced in the physiological, biochemical and anatomical characteristics of Vicia faba by the exogenous application of proline under seawater stressS. Afr. J. Bot.935463

    • Search Google Scholar
    • Export Citation
  • DhindsaR.S.Plumb-DhindsaP.ThorpeT.A.1981Leaf senescence: Correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalaseJ. Expt. Bot.3293101

    • Search Google Scholar
    • Export Citation
  • FagernessM.J.YelvertonF.H.2001Plant growth regulator and mowing height effects on seasonal root growth of penncross creeping bentgrassCrop Sci.4119011905

    • Search Google Scholar
    • Export Citation
  • GrieveC.GrattanS.1983Rapid assay for determination of water soluble quaternary ammonium compoundsPlant Soil70303307

  • HeC.ZhangW.GaoQ.YangA.HuX.ZhangJ.2011Enhancement of drought resistance and biomass by increasing the amount of glycine betaine in wheat seedlingsEuphytica177151167

    • Search Google Scholar
    • Export Citation
  • HouP.MaJ.ZhaoP.ZhangH.ZhaoH.LiuH.ZhaoY.WangY.MaJ.ZhaoP.2013Effects of betaine on chloroplast protective enzymes and psbA gene expression in wheat seedlings under drought stressActa Agronomica Sinica3913191324

    • Search Google Scholar
    • Export Citation
  • HuL.WangZ.HuangB.2010Diffusion limitations and metabolic factors associated with inhibition and recovery of photosynthesis from drought stress in a C3 perennial grass speciesPhysiol. Plant.13993106

    • Search Google Scholar
    • Export Citation
  • HuangB.DacostaM.JiangY.2014Research advances in mechanisms of turfgrass tolerance to abiotic stresses: From physiology to molecular biologyCrit. Rev. Plant Sci.33141189

    • Search Google Scholar
    • Export Citation
  • Hussain WaniS.Brajendra SinghN.HaribhushanA.Iqbal MirJ.2013Compatible solute engineering in plants for abiotic stress tolerance: Role of glycine betaineCurr. Genomics14157165

    • Search Google Scholar
    • Export Citation
  • IuchiS.KobayashiM.TajiT.NaramotoM.SekiM.KatoT.TabataS.KakubariY.Yamaguchi-ShinozakiK.ShinozakiK.2001Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in ArabidopsisPlant J.27325333

    • Search Google Scholar
    • Export Citation
  • JiaX.-J.DongL.-H.DingC.-B.LiX.YuanM.2013Effects of drought stress on reactive oxygen species and their scavenging systems in Chlorophytum capense var. medio-pictum leafActa Prataculturae Sinica22248255

    • Search Google Scholar
    • Export Citation
  • LarrainzarE.MolenaarJ.A.WienkoopS.Gil-QuintanaE.AlibertB.LimamiA.M.Arrese-IgorC.GonzálezE.M.2014Drought stress provokes the down-regulation of methionine and ethylene biosynthesis pathways in M. edicago truncatula roots and nodulesPlant Cell Environ.3720512063

    • Search Google Scholar
    • Export Citation
  • LiZ.-G.GongM.2005Improvement of measurement method of polyphenol oxidase activities in plantJ. Yunnan Normal Univ14449

  • LouY.YangY.HuL.LiuH.XuQ.2015Exogenous glycinebetaine alleviates the detrimental effect of Cd stress on perennial ryegrassEcotoxicology2413301340

    • Search Google Scholar
    • Export Citation
  • LuoD.NiuX.YuJ.YanJ.GouX.LuB.-R.LiuY.2012Rice choline monooxygenase (OsCMO) protein functions in enhancing glycine betaine biosynthesis in transgenic tobacco but does not accumulate in rice (Oryza sativa L. ssp. japonica)Plant Cell Rpt.3116251635

    • Search Google Scholar
    • Export Citation
  • MaQ.-Q.WangW.LiY.-H.LiD.-Q.ZouQ.2006Alleviation of photoinhibition in drought-stressed wheat (Triticum aestivum) by foliar-applied glycinebetaineJ. Plant Physiol.163165175

    • Search Google Scholar
    • Export Citation
  • MaQ.ZouQ.LiY.LiD.WangW.2004Amelioration of the water status and improvement of the anti-oxidant enzyme activities by exogenous glycinebetaine in water-stressed wheat seedlingsZuo Wu Xue Bao30321328

    • Search Google Scholar
    • Export Citation
  • MalekzadehP.2015Influence of exogenous application of glycinebetaine on antioxidative system and growth of salt-stressed soybean seedlings (Glycine max L.)Physiol. Mol. Biol. Plants21225232

    • Search Google Scholar
    • Export Citation
  • MaziahM.TehC.2016Exogenous application of glycine betaine alleviates salt induced damages more efficiently than ascorbic acid in in vitro rice shootsAustral. J. Basic Appl. Sci.105865

    • Search Google Scholar
    • Export Citation
  • MoffattB.A.WeretilnykE.A.2001Sustaining S-adenosyl-l-methionine-dependent methyltransferase activity in plant cellsPhysiol. Plant.113435442

    • Search Google Scholar
    • Export Citation
  • PelegZ.BlumwaldE.2011Hormone balance and abiotic stress tolerance in crop plantsCurr. Opin. Plant Biol.14290295

  • RathinasabapathiB.2000Metabolic engineering for stress tolerance: Installing osmoprotectant synthesis pathwaysAnn. Bot. (Lond.)86709716

    • Search Google Scholar
    • Export Citation
  • RazaM.SaleemM.ShahG.KhanI.RazaA.2014Exogenous application of glycinebetaine and potassium for improving water relations and grain yield of wheat under droughtJ. Soil Sci. Plant Nutr.14348364

    • Search Google Scholar
    • Export Citation
  • ReddyA.R.ChaitanyaK.V.VivekanandanM.2004Drought-induced responses of photosynthesis and antioxidant metabolism in higher plantsJ. Plant Physiol.16111891202

    • Search Google Scholar
    • Export Citation
  • SaneokaH.NagasakaC.HahnD.T.YangW.-J.PremachandraG.S.JolyR.J.RhodesD.1995Salt tolerance of glycinebetaine-deficient and -containing maize linesPlant Physiol.107631638

    • Search Google Scholar
    • Export Citation
  • TurgeonA.J.1991Turfgrass management. Prentice-Hall Inc. Upper Saddle River NJ

  • WangK.L.-C.LiH.EckerJ.R.2002Ethylene biosynthesis and signaling networksPlant Cell14S131S151

  • WangG.-P.TianF.-X.ZhangM.WangW.2014The overaccumulation of glycinebetaine alleviated damages to PSII of wheat flag leaves under drought and high temperature stress combinationActa Physiol. Plant.3627432753

    • Search Google Scholar
    • Export Citation
  • WarnkeS.2003Creeping bentgrass (Agrostis stolonifera L.) p. 175–185. Turfgrass biology genetics and breeding. Wiley Hoboken NJ

  • YamamotoY.AminakaR.YoshiokaM.KhatoonM.KomayamaK.TakenakaD.YamashitaA.NijoN.InagawaK.MoritaN.2008Quality control of photosystem II: Impact of light and heat stressesPhotosynth. Res.98589608

    • Search Google Scholar
    • Export Citation
  • YangZ.YuJ.MerewitzE.HuangB.2012Differential effects of abscisic acid and glycine betaine on physiological responses to drought and salinity stress for two perennial grass speciesJ. Amer. Soc. Hort. Sci.13796106

    • Search Google Scholar
    • Export Citation
  • ZhangL.GaoM.ZhangL.LiB.HanM.AlvaA.K.AshrafM.2013Role of exogenous glycinebetaine and humic acid in mitigating drought stress-induced adverse effects in Malus robusta seedlingsTurk. J. Bot.37920929

    • Search Google Scholar
    • Export Citation
  • ZhangJ.KirkhamM.1996Enzymatic responses of the ascorbate-glutathione cycle to drought in sorghum and sunflower plantsPlant Sci.113139147

    • Search Google Scholar
    • Export Citation
  • ZhangN.SiH.-J.WenG.DuH.-H.LiuB.-L.WangD.2011Enhanced drought and salinity tolerance in transgenic potato plants with a BADH gene from spinachPlant Biotechnol. Rpt.57177

    • Search Google Scholar
    • Export Citation
  • ZhaoP.WangL.ZhaoH.LiangS.LvS.QuX.WangY.2010Regulation of exogenous salicylic acid on expression of chloroplast gene psbA in wheat leaves under heat and high irradiancePlant Physiol. Commun..46537540

    • Search Google Scholar
    • Export Citation
  • ZhouL.YanP.Xing-YuanS.2013Physiological responses of white clover by different leaf types associated with anti-oxidative enzyme protection and osmotic adjustment under drought stressActa Prataculturae Sinica2202257263

    • Search Google Scholar
    • Export Citation
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