Micropropagation and Ex Vitro Rooting of Wolfberry

in HortScience

An accurate protocol for the in vitro propagation of a commercial wolfberry (Lycium barbarum L.) cv. Nixia 1 has been developed through axillary shoot proliferation. Driver and Kuniyuki Walnut (DKW) medium supplemented with 6-benzylaminopurine (BAP; 0.5 mg/L) and sucrose 3% w/v gave the best results compared with other basal media tested, with significantly improved production of multiple shoots direct from nodal segment explants, resulting in an average of 6.73 shoots/explant with an average of 7.45 nodes/shoot that would potentially form new explants. Rooting of shoot explants was carried out both in vitro and ex vitro with 0.5 and 1 mg/L of indole-3-butyric acid (IBA), with or without adding putrescine (160 mg/L). In all cases, rooting efficiency resulted very high, and putrescine was effective only when combined with a low concentration of auxin. Plantlets were hardened off in jiffy pots under greenhouse conditions, with a survival rate of more than 90%. Ex vitro rooting, performed by dipping in an aqueous solution of IBA 100 mg/L, is the preferred technique not only because rooting and acclimatization are very high but also reducing micropropagation to one phase is more economical.

Contributor Notes

The research was partially supported by MIUR (MInistry for education, University and Research), Law 232/2016, “Department of excellence” and by funding of Tuscia University DAFNE.RSA16SIL (Ricerca Scientifica Ateneo Silvestri). We thank Antonella Minandri for her work and well-qualified assistance in in vitro culture.

Corresponding author. E-mail: silvestri.c@unitus.it.

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    In vitro shoot culture of wolfberry cv. Nixia 1. Shown are 0.1, 0.5, 1, and 2 mg/L of (A–D) 6-benzylaminopurine (BAP), (E–H) N6-(2-Isopentenyl)adenine (2-iP), and (I-L) zeatin (ZR).

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    In vitro shoot culture of wolfberry cv. Nixia 1 on media Murashige and Skoog (MS), Quoirin and Lepoivre (QL), Woody Plant Medium (WPM), and Driver and Kuniyuki Walnut (DKW) in order from left to right.

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    (A) Plantlets of wolfberry rooted ex vitro. (B) Well-developed adventitious rootlets after 3 weeks of acclimatization.

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