Protective Netting Improves Leaf-level Photosynthetic Light Use Efficiency in ‘Honeycrisp’ Apple Under Heat Stress

in HortScience

Globally, apple production often occurs in semiarid climates characterized by high summer temperatures and solar radiation. Heat stress events occur regularly during the growing season in these regions. For example, in the semiarid eastern half of Washington State, historic weather data show that, on average, 33% of the days during the growing season exceed 30 °C. To mediate some of the effects of heat stress, protective netting (PN) can be used to reduce the occurrence of fruit sunburn. However, the impacts of reduced solar radiation in a high light environment on light-use efficiency and photosynthesis are poorly understood. We sought to understand the ecophysiological response of apple (Malus domestica Borkh. cv. Honeycrisp) under blue photoselective PN during days with low (26.6 °C), moderate (33.7 °C), or high (38.1 °C) ambient temperatures. Two treatments were evaluated; an uncovered control and blue photoselective PN. Maximum photochemical efficiency of PSII, or photosystem II (Fv/Fm) was significantly greater at all measurement times under blue photoselective PN compared with the control on days with high ambient temperatures. Fv/Fm dropped below 0.79, which is considered the threshold for stress, at 1000 hr in the control and at 1200 hr under blue photoselective PN on a day with high ambient temperature. On days with low or moderate ambient temperatures, Fv/Fm was significantly greater under blue photoselective PN at 1400 hr, which coincided with the peak in solar radiation. ‘Honeycrisp’ apple exhibited dynamic photoinhibition as shown by the diurnal decline in Fv/Fm. Quantum photosynthetic yield of PSII (ΦPSII) was also generally greater under blue photoselective PN compared with the control for days with moderate or high ambient temperatures. Photochemical reflectance index (ΔPRI), the difference in reflectance between a stress-responsive and nonstress-responsive wavelength, was greater under PN compared with the control on the day with high ambient temperatures, with no differences observed under low or moderate ambient temperatures. Leaf gas exchange did not show noticeable improvement under blue photoselective netting when compared with the control despite the improvement in leaf-level photosynthetic light use efficiency. In conclusion, PN reduced incoming solar radiation, improved leaf-level photosynthetic light use efficiency, and reduced the symptoms of photoinhibition in a high-light, arid environment.

Contributor Notes

We thank Katie Mullin, Jordan Briggs, Michelle Reid, and Chelsea Hill from the Kalcsits Lab at the WSU Tree Fruit Research and Extension Center for assistance with data collection. Funding for the research was provided by the Washington State Department of Agriculture Specialty Crop Block Grant (K1771) and the Washington Tree Fruit Research Commission (AP-15-104A). The contribution of anonymous reviewers who greatly improved the quality of this manuscript is acknowledged.

Corresponding authors. E-mail: lee.kalcsits@wsu.edu or giverson.mupambi@wsu.edu.

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    Above-canopy photosynthetic photon flux density (PPFD) under blue photoselective protective netting and an uncovered control measured during days with low (26.6 °C), moderate (33.7 °C), or high (38.1 °C) ambient temperature at Washington State University Tree Fruit Research and Extension Center, Wenatchee, WA.

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    Above-canopy ambient temperature under blue photoselective protective netting and an uncovered control measured during days with low (26.6 °C), moderate (33.7 °C), or high (38.1 °C) ambient temperature at Washington State University Tree Fruit Research and Extension Center, Wenatchee, WA.

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    Diurnal change in maximum photochemical efficiency of photosystem II (Fv/Fm) of ‘Honeycrisp’ apple during days with low (26.6 °C), moderate (33.7 °C), or high (38.1 °C) ambient temperature under blue photoselective protective netting and an uncovered control. Means ± se at each sampling time point were separated by a two-sample t test (ns = not significant, *P ≤ 0.05, **P ≤ 0.001, ***P ≤ 0.0001).

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    Diurnal change in quantum photosynthetic yield of photosystem II (ΦPSII) of ‘Honeycrisp’ apple during days with moderate (33.7 °C) or high (38.1 °C) ambient temperature under blue photoselective protective netting and an uncovered control. Means ± se at each sampling time point were separated by a two-sample t test (ns = not significant, *P ≤ 0.05, **P ≤ 0.001, ***P ≤ 0.0001).

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    Diurnal change in photochemical reflectance index (ΔPRI) of ‘Honeycrisp’ apple during days with low (26.6 °C), moderate (33.7 °C), or high (38.1 °C) ambient temperature under blue photoselective protective netting and an uncovered control. Means ± se at each sampling time point were separated by a two-sample t test (ns = not significant, *P ≤ 0.05, **P ≤ 0.001, ***P ≤ 0.0001).

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    Diurnal change in leaf water potential (Ψl) of ‘Honeycrisp’ apple during days with low (26.6 °C), moderate (33.7 °C), or high (38.1 °C) air temperature under blue photoselective protective netting compared with an uncovered control. Means ± se at each sampling time point were separated by a two-sample t test (ns = not significant, *P ≤ 0.05, **P ≤ 0.0001, ***P ≤ 0.001).

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    Midday stem water potential (Ψmd) of ‘Honeycrisp’ apple during days with low (26.6 °C), moderate (33.7 °C), or high (38.1 °C) ambient temperature under blue photoselective PN compared with an uncovered control. Means ± se at each sampling time point were separated by a two-sample t test (ns = not significant, *P ≤ 0.05, **P ≤ 0.001, ***P ≤ 0.0001).

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