Selection of a Suitable Pollinizer for ‘Summer Fantasia’ Plum

in HortScience

Being self-incompatible, most Asian plums should be planted with a compatible pollinizer to produce fruits. Therefore, the selection of an adequate pollinizer is essential when new plum cultivars are released. To select a suitable pollinizer for ‘Summer Fantasia’ plum, the S genotype, cross-compatibility, pollen viability, and flowering time of five candidate cultivars were evaluated. Using polymerase chain reaction (PCR) analysis, the S genotype of ‘Summer Fantasia’ was determined as SaSc, which was expected to be compatible with other S genotypes. To test cross-compatibility, the trees were covered with caging net to prevent unintended pollination, and pistils were hand pollinated without emasculation. Fruit set percentage was calculated 10 weeks after pollination. The fruit set percentage resulting from the cross between ‘Summer Fantasia’ and ‘Taiyo’ (SbSc) was 13.8%, whereas that resulting from other combinations was less than 5.0%. Pollen germination percentage was investigated to monitor pollen viability; it varied yearly among cultivars, although ‘Formosa’ (10.6%) and ‘Taiyo’ (13.8%) showed the highest pollen germination percentages among the cultivars. When averaged over three years and two locations, ‘Summer Fantasia’ bloomed 2–3 days after ‘Akihime’, ‘Formosa’, ‘Oishiwase’, and ‘Purple Queen’. Blooming period of ‘Summer Fantasia’ and ‘Taiyo’ overlapped almost entirely. Overall, the results indicated that ‘Taiyo’ was the most suitable pollinizer for ‘Summer Fantasia’.

Contributor Notes

Funding for this research was provided by a project (PJ010228202) of Rural Development Administration, Republic of Korea.

Corresponding author. E-mail:

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    Results of the polymerase chain reaction analysis of plum cultivars using the S-RNase gene-specific primer pair Pru-C2 and PCE-R. M, molecular size marker; 1, ‘Akihime’ (Sh-); 2, ‘Formosa’ (SbSd); 3, ‘Oishiwase’ (ScSd); 4, ‘Purple Queen’ (SbSc); 5, ‘Summer Fantasia’ (SaSc); 6, ‘Taiyo’ (SbSc).

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    Results of the polymerase chain reaction analysis of plum cultivars using the S-RNase gene-specific primer pair Pru-C2 and Pru-C6R. M, molecular size marker; 1, ‘Akihime’ (Sh-); 2, ‘Formosa’ (SbSd); 3, ‘Oishiwase’ (Sd-); 4, ‘Purple Queen’ (Sb-); 5, ‘Summer Fantasia’ (Sa-); 6, ‘Taiyo’ (SbSc).

Article References

AldermanW.H.AngeloE.1932Self and cross sterility in plum hybridsProc. Amer. Soc. Hort. Sci.29118121

BeppuK.KomatsuN.YamaneH.YaegakiH.YamaguchiM.TaoR.KataokaI.2005Se-haplotype confers self-compatibility in Japanese plum (Prunus salicina Lindl.)J. Hort. Sci. Biotechnol.80760764

BeppuK.KumaiM.YamaneH.TaoR.KataokaI.2012Molecular and genetic analysis of the S-haplotype of the self-compatible Japanese plum (Prunus salicina Lindl.) ‘Methley’J. Hort. Sci. Biotechnol.87493498

BeppuK.TakemotoY.YamaneH.YaegakiH.YamaguchiM.KataokaI.TaoR.2003Determination of S-haplotypes of Japanese plum (Prunus salicina Lindl.) cultivars by PCR and cross-pollination testsJ. Hort. Sci. Biotechnol.78315318

BeppuK.YamaneH.YaegakiH.YamaguchiM.KataokaI.TaoR.2002Diversity of S-RNase genes and S-haplotypes in Japanese plum (Prunus salicina Lindl.)J. Hort. Sci. Biotechnol.77658664

BesshoH.WadaM.KudoK.InomataY.IwanamiH.AbeK.SoejimaJ.KomoriS.SuzukiA.2009Selection of crabapple pollinizers for ‘Fuji’ and ‘Tsugaru’ appleJ. Amer. Pomol. Soc.63213

BroothaertsW.Van NerumI.KeulemansJ.2004Update on and review of the incompatibility (S-) genotypes of apple cultivarsHortScience39943947

ĆalićD.DevrnjaN.KostićI.KostićM.2013Pollen morphology, viability, and germination of Prunus domestica cv. PožegačaSci. Hort.155118122

ChungK.H.YoonS.K.NamE.Y.YunI.K.ParkJ.M.LeeS.C.ChungD.S.2013The guide of agricultural technique Plum and mume (in Korean). Rural Development Administration Suwon Republic of Korea

CrasswellerR.M.FerreeD.C.NicholsL.P.1980Flowering crab apples as potential pollinizers for commercial apple cultivarsJ. Amer. Soc. Hort. Sci.105475477

DuY.H.ZhangS.L.JiangX.T.WuJ.2006Characteristics of pollen germination and pollen tube growth of Prunus mume in vitroXibei Zhiwu Xuebao2618461852

FloryW.S.TomesM.L.1943Studies of plum pollen, its appearance and germinationJ. Agr. Res.67337342

GoldwayM.ShaiO.YehudaH.MatityahuA.SternR.A.1999‘Jonathan’ apple is a lower-potency pollenizer of ‘Topred’ than ‘Golden Delicious’ due to partial S-allele incompatibilityJ. Hort. Sci. Biotechnol.74381385

GoldwayM.SternR.A.ZisovichA.H.ShafirS.2005Application of S-RNase allele molecular analysis as a means for elucidating low yields in the pear orchardActa Hort.671137142

GuerraM.E.RodrigoJ.2015Japanese plum pollination: A reviewSci. Hort.197674686

GuerraM.E.RodrigoJ.López-CorralesM.WűnschA.2009S-RNase genotyping and incompatibility group assignment by PCR and pollination experiments in Japanese plumPlant Breed.128304311

GuerraM.E.WünschA.López-CorralesM.RodrigoJ.2010Flower emasculation as the cause for lack of fruit set in Japanese plum crossesJ. Amer. Soc. Hort. Sci.135556562

HalászJ.HegedűsA.GyörgyZ.PállingerÉ.TóthM.2011S-genotyping of old apple cultivars from the Carpathian basin: Methodological, breeding and evolutionary aspectsTree Genet. Genomes711351145

HalászJ.HegedűsA.HermánR.Stefanovits-BányaiÉ.PedrycA.2005New self-incompatibility alleles in apricot (Prunus armeniaca L.) revealed by stylar ribonuclease assay and S-PCR analysisEuphytica1455766

HalászJ.HegedűsA.SzabóZ.NyékiJ.PedrycA.2007DNA-based S-genotyping of Japanese plum and pluot cultivars to clarify incompatibility relationshipsHortScience424650

HartmannW.NeumüllerM.2009Plum breeding p. 161–231. In: S.M. Jain and P.M. Priyadarshan (eds.). Breeding plantation tree crops: Temperate species. Springer New York

HedhlyA.HormazaJ.I.HerreroM.2004Effect of temperature on pollen tube kinetics and dynamics in sweet cherry, Prunus avium (Rosaceae)Amer. J. Bot.91558564

HedhlyA.HormazaJ.I.HerreroM.2005The effect of temperature on pollen germination, pollen tube growth, and stigmatic receptivity in peachPlant Biol.7476483

IkedaK.WatariA.UshijimaK.YamaneH.HauckN.R.IezzoniA.F.TaoR.2004Molecular markers for the self-compatible S4′-haplotype, a pollen-part mutation in sweet cherry (Prunus avium L.)J. Amer. Soc. Hort. Sci.129724728

IshimizuT.InoueK.ShimonakaM.SaitoT.TeraiO.NoriokaS.1999PCR-based method for identifying the S-genotypes of Japanese pear cultivarsTheor. Appl. Genet.98961967

JanssensG.A.GoderisI.J.BroekaertW.F.BroothaertsW.1995A molecular method for S-allele identification in apple based on allele-specific PCRTheor. Appl. Genet.91691698

JunJ.H.KwonJ.H.ChungK.H.2007Identification of self-incompatibility genotypes in Japanese plum (Prunus salicina Lindl.) by polyemrase chain reaction and cross-pollination testHort. Environ. Biotechnol.48228234

JunJ.H.KwonJ.H.DoK.R.YunI.K.MaK.B.KimJ.B.2010Analysis of ploidy and S-alleles of ‘Kiyo’ a Japanese plumKor. J. Hort. Sci. Technol.2898102

JunJ.H.NamE.Y.ChungK.H.YunS.K.KwonJ.H.YunI.K.KwackY.B.KimS.J.ChoK.H.2015‘Summer Fantasia’ Japanese plumHortScience50932933

KangI.K.LeeG.J.KimM.J.KwonS.I.PeakP.Y.ChoiD.G.2002Selection of crabapple as pollinizers for major apple cultivars in apple orchardKor. J. Hort. Sci. Technol.20330334

KaoT.TsukamotoT.2004The molecular and genetic bases of S-RNase-based self-incompatibilityPlant Cell16Suppl.7283

Korea Meteorological Administration2011Climatological normals of Korea. Korea Meteorological Administration. Seoul Republic of Korea

KoyuncuF.TosunF.2005Evaluation of pollen viability and germinating capacity of some sweet cherry cultivars grown in Isparta, TurkeyActa Hort.7957174

LaRueJ.H.NortonM.V.1989Japanese plum pollination p. 49–55. In: J.H. LaRue and R.S. Johnson (eds). Peaches plums and nectarines: Growing and handling for fresh market. Univ. of California Oakland CA

McGranahanG.H.VoyiatzisD.G.CatlinP.B.PolitoV.S.1994High pollen loads can cause pistillate flower abscission in walnutJ. Amer. Soc. Hort. Sci.119505509

NikolićD.MilatovićD.2010Examining self-compatibility in plum (Prunus domestica L.) by fluorescence microscopyGenetika42387396

NyékiJ.SzabóZ.1996Fruit set of plum cultivars under Hungarian ecological conditionsActa Hort.423185192

NyékiJ.SzabóZ.1997Cross-incompatibility in stone fruitsActa Hort.437213218

OkieW.R.BlackburnB.2011Increasing chilling reduces heat requirement for floral budbreak in peachHortScience46245252

OkieW.R.HancockJ.F.2008Plums p. 337–357. In: J.F. Hancock (ed.). Temperate fruit crop breeding: Germplasm to genomics. Springer Netherlands

OkieW.R.WeinbergerH.1996Plums p. 559–607. In: J. Janick and J.N. Moore (eds.). Fruit breeding. Vol. I. Tree and tropical fruits. Wiley New York NY

R Core Team2014R: A language and environment for statistical computing. R foundation for statistical computing Vienna Austria. <>

SapirG.SternR.A.ShafirS.GoldwayM.2008aFull compatibility is superior to semi-compatibility for fruit set in Japanese plum (Prunus salicina Lindl.) cultivarsSci. Hort.116394398

SapirG.SternR.A.ShafirS.GoldwayM.2008bS-RNase based S-genotyping of Japanese plum (Prunus salicina Lindl.) and its implication on the assortment of cultivar-couples in the orchardSci. Hort.118813

SchneiderD.SternR.A.EisikowitchD.GoldwayM.2001Analysis of S-alleles by PCR for determination of compatibility in the ‘Red Delicious’ apple orchardJ. Hort. Sci. Biotechnol.76596600

SharafiY.2011An investigation on the pollen germination and tube growth in some Prunus persica genotypes and cultivarsAfr. J. Microbiol. Res.520032007

TamuraM.UshijimaK.SassaH.HiranoH.TaoR.GradzielT.M.DandekarA.M.2000Identification of self-incompatibility genotypes of almond by allele-specific PCR analysisTheor. Appl. Genet.101344349

TaoR.HabuT.YamaneH.SugiuraA.IwamotoK.2000Molecular markers for self-compatibility in Japanese apricot (Prunus mume)HortScience3511211123

TaoR.IezzoniA.F.2010The S-RNase-based gametophytic self-incompatibility system in Prunus exhibits distinct genetic and molecular featuresSci. Hort.124423433

TaoR.YamaneH.SugiuraA.MurayamaH.SassaH.MoriH.1999Molecular typing of S-alleles through identification, characterization and cDNA cloning for S-RNases in sweet cherryJ. Amer. Soc. Hort. Sci.124224233

VilanovaS.RomeroC.AbernathyD.AbbottA.G.BurgosL.LlacerG.BadenesM.L.2003Construction and application of a bacterial artificial chromosome (BAC) library of Prunus armeniaca L. for the identification of clones linked to the self-incompatibility locusMol. Genet. Genomics269685691

VilanovaS.RomeroC.LlácerG.BadenesM.L.BurgosL.2005Identification of self-(in)compatibility alleles in apricot by PCR and sequence analysisJ. Amer. Soc. Hort. Sci.130893898

WatariA.HanadaT.YamaneH.EsumiT.TaoR.YaegakiH.YamaguchiM.BeppuK.KataokaI.2007A low transcriptional level of Se-RNase in the Se-haplotype confers self-compatibility in Japanese plumJ. Amer. Soc. Hort. Sci.132396406

WaughF.A.1896Problems in plum pollination. Annu. Rpt. VermontAgr. Expt. Sta.108798

YaegakiH.ShimadaT.MoriguchiT.HayamaH.HajiT.YamaguchiM.2001Molecular characterization of S-RNase genes and S-genotypes in the Japanese apricot (Prunus mume Sieb. et Zucc.)Sex. Plant Reprod.13251257

YamaneH.TaoR.SugiuraA.1999Identification and cDNA cloning for S-RNases in self-incompatible Japanese plum (Prunus salicina Lindl. cv. Sordum)Plant Biotechnol.16389396

YamaneH.TaoR.SugiuraA.HauckN.R.IezzoniA.F.2001Identification and characterization of S-RNases in tetraploid sour cherry (Prunus cerasus)J. Amer. Soc. Hort. Sci.126661667

ZhangS.L.HuangS.X.KitashibaH.NishioT.2007Identification of S-haplotype-specific F-box gene in Japanese plum (Prunus salicina Lindl.)Sex. Plant Reprod.2018

ZisovichA.SternR.A.ShafirS.GoldwayM.2005Fertilisation efficiency of semi- and fully-compatible European pear (Pyrus communis L.) cultivarsJ. Hort. Sci. Biotechnol.80143146

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