Initiation and Cytological Aspects of Somatic Embryogenesis in Dendrobium candidum Wall ex Lindl.

in HortScience

To find the characteristics of somatic embryogenesis of orchids and elucidate the mechanism, we had previously established an efficient plant regeneration system via somatic embryogenesis in Dendrobium candidum Wall ex Lindl. In this study, a detailed cytological investigation was carried out on the initiation and developmental process of somatic embryogenesis. Based on our observations, the somatic embryogenesis in D. candidum originated from the transition of an embryonic callus cell to the initial somatic embryo cell, and the somatic embryos initiated from those cells. During the transition process, condensation and devacuolation successively occurred in the cytoplasm of the embryonic callus cells, giving rise to the formation of a typical initial somatic embryo cell with dense cytoplasm and a clear nucleus. One of the two pathways in somatic embryogenesis is the single-cell-derived somatic embryo which is generated from an inner initial somatic embryo cell in embryonic callus and develops into a globular somatic embryo in a way similar to zygotic embryogenesis and then keeps developing into a protocorm-like body (PLB). The other is a multiple-cell-derived somatic embryo which is generated from peripheral grouped initial somatic cells in embryonic calli and directly forms globular embryo or multicellular somatic proembryo, lacking the typical early stages of embryogenesis. Both pathways were observed in the somatic embryogenesis system, indicating that the culture system in D. candidum can be a useful tool for investigating the mechanisms underlying orchid embryogenesis.

Contributor Notes

This work was supported by the Grant (No. 31371232) from the National Natural Science Foundation of the People’s Republic of China.

Co-first authors.

Corresponding author. E-mail: wanjunwang@home.swjtu.edu.cn.

  • ArdittiJ.ClementsM.A.FastG.HadleyG.NishimuraG.ErnestR.1982Orchid seed germination and seedling culture a manual p. 243–370. In: J. Arditti (ed.). Orchid biology-reviews and perspectives II. Cornell University Press Ithaca NY

  • ArdittiJ.1992Fundamentals of orchid biology. Wiley New York NY

  • BegumA.A.TamakiM.TaharaM.KakoS.J.1994Somatic embryogenesis in Cymbidium through in vitro culture of inner tissue of protocorm-like bodiesJ. Jpn. Soc. Hort. Sci.63419427

    • Search Google Scholar
    • Export Citation
  • ChenJ.T.ChangW.C.2000Efficient plant regeneration through somatic embryogenesis from callus cultures of Oncidium (Orchidaceae)Plant Sci.1608793

    • Search Google Scholar
    • Export Citation
  • ChenJ.T.ChangW.C.2006Direct somatic embryogenesis and plant regeneration from leaf explants of Phalaenopsis amabilisBiol. Plant.50169173

    • Search Google Scholar
    • Export Citation
  • ChungJ.D.KimH.Y.SuhJ.H.KwonO.C.KimC.K.2006Direct somatic embryogenesis from thin-section leaf explant of Phalaenopsis hybridsHortScience4110661067

    • Search Google Scholar
    • Export Citation
  • DutraD.KaneM.E.RichardsonL.2009Asymbiotic seed germination and in vitro seedling development of Cyrtopodium punctatum: A propagation protocol for an endangered Florida native orchidPlant Cell Tissue Organ. Cult.96235243

    • Search Google Scholar
    • Export Citation
  • DutraD.StewartS.L.KauthJ.JohnsonT.R.PhilmanN.KaneM.E.RichardsonL.2007Asymbiotic seed germination of the threatened orchid Bletia puipurea in FloridaIn Vitro Cell. Dev. Biol. Anim.43S45

    • Search Google Scholar
    • Export Citation
  • GillesL.DenisB.JoachimV.1997Morphogenesis of the protocorm of Cypripedium acaule (Orchidaceae)Plant Syst. Evol.2055372

  • HigashiK.ShiotaH.KamadaH.1998Patterns of expression of the genes for glutamine synthetase isoforms during somatic and zygotic embryogenesis in carrotPlant Cell Physiol.39418424

    • Search Google Scholar
    • Export Citation
  • HuanL.V.TakamuraT.TanakaA.2004Callus formation and plant regeneration from callus through somatic embryo structures in Cymbidium orchidPlant Sci.16614431449

    • Search Google Scholar
    • Export Citation
  • HuangB.Q.YeX.L.YeungE.C.ZeeS.Y.1998Embryology of Cymbidium sinense: the microtubule organization of early embryosAnn. Bot.81741750

  • Ikeda-IwaiM.SatohS.KamadaH.2002Establishment of a reproducible tissue culture system for the induction of Arabidopsis somatic embryosJ. Expt. Bot.5315751580

    • Search Google Scholar
    • Export Citation
  • IshiiY.TakamuraT.M.GoiM.TanakaM.1998Callus induction and somatic embryogenesis of PhalaenopsisPlant Cell Rpt.17446450

  • JasikJ.SalajovaT.SalajJ.1995Developmental anatomy and ultrastructure of early somatic embryos in European black pine (Pinus nigra Arn.)Protoplasma185205211

    • Search Google Scholar
    • Export Citation
  • JensenW.A.1962Botanical histochemistry. Freeman San Francisco CA

  • LeeE.K.ChoD.Y.SohW.Y.2001Enhanced production and germination of somatic embryos by temporary starvation in tissue cultures of Daucus carotaPlant Cell Rpt.20408415

    • Search Google Scholar
    • Export Citation
  • LiY.WangC.L.GuoS.X.YangJ.S.XiaoP.G.2008Two new compounds from Dendrobium candidumChem. Pharm. Bull. (Tokyo)5614771479

  • LiY.WangC.L.WangY.J.GuoS.X.YangJ.S.ChenX.M.XiaoP.G.2009aThree new bibenzyl derivatives from Dendrobium candidumChem. Pharm. Bull. (Tokyo)57218219

    • Search Google Scholar
    • Export Citation
  • LiY.WangC.L.WangY.J.GuoS.X.YangJ.S.ChenX.M.XiaoP.G.2009bFour new bibenzyl derivatives from Dendrobium candidumChem. Pharm. Bull. (Tokyo)57997999

    • Search Google Scholar
    • Export Citation
  • LuoY.KoopH.U.1997Somatic embryogenesis in cultured immature zygotic embryos and leaf protoplasts of Arabidopsis thaliana ecotypesPlanta202387396

    • Search Google Scholar
    • Export Citation
  • MaheswaranG.WilliamsE.G.1985Origin and development of somatic embryoids formed directly on immature embryos of Trifolium repens in vitroAnn. Bot.56619630

    • Search Google Scholar
    • Export Citation
  • MenesesA.FloresD.MunozM.ArrietaG.EspinozaA.M.2005Effect of 2,4-d, hydric stress and light on indica rice (Oryza sativa) somatic embryogenesisRev. Biol. Trop.53361368

    • Search Google Scholar
    • Export Citation
  • NagmaniR.BecwarM.R.WannS.R.1987Single-cell origin and development of somatic embryos in Picea abies (L.) Karst. (Norway spruce) and P. glauca (Moench) Voss (white spruce)Plant Cell Rpt.6157159

    • Search Google Scholar
    • Export Citation
  • NishiwakiM.FujinoK.KodaY.MasudaK.KikutaY.2000Somatic embryogenesis induced by the simple application of abscisic acid to carrot (Daucus carota L.) seedlings in culturePlanta211756759

    • Search Google Scholar
    • Export Citation
  • NomuraK.KomamineA.1985Identification and isolation of single cells that produce somatic embryos at a high frequency in a carrot suspension culturePlant Physiol.79988991

    • Search Google Scholar
    • Export Citation
  • RavalM.ChattooB.B.1993Role of media constituents and proline in callus growth, somatic embryogenesis and regeneration of Oryza sativa cv IndicaIndian J. Expt. Biol.31600603

    • Search Google Scholar
    • Export Citation
  • RasmussenH.N.1995Terrestrial orchids from seed to mycotrophic plant. Cambridge University Press New York NY

  • ShiauY.J.NalawadeS.M.HsiaC.N.MulabagalV.TsayH.S.2005In vitro propagation of the Chinese medicinal plant, Dendrobium candidum Wall. Ex Lindl., from axenic nodal segmentsIn Vitro Cell. Dev. Biol. Plant41666670

    • Search Google Scholar
    • Export Citation
  • StewartS.L.KaneM.E.2005In vitro seed germination and seedling development of Habenaria mocroceratitis, an endangered terrestrial orchidIn Vitro Cell. Dev. Biol. Anim.4127a

    • Search Google Scholar
    • Export Citation
  • StewartS.L.KaneM.E.2006Asymbiotic seed germination and in vitro seedling development of Habenaria macroceratitis (Orchidaceae), a rare Florida terrestrial orchidPlant Cell Tissue Organ. Cult.86147158

    • Search Google Scholar
    • Export Citation
  • SwamyB.G.L.1949Embryological studies in the Orchidaceae. II. EmbryologyAmer. Midl. Nat.41184201

  • TsavkelovaE.A.CherdyntsevaT.A.KlimovaS.Y.ShestakovA.I.BotinaS.G.NetrusovA.I.2007Orchid-associated bacteria produce indole-3-acetic acid, promote seed germination, and increase their microbial yield in response to exogenous auxinArch. Microbiol.188655664

    • Search Google Scholar
    • Export Citation
  • WilliamsE.G.MaheswaranG.1986Somatic embryogenesis: factors influencing coordinated behaviour of cells as an embryogenic groupAnn. Bot.57443462

    • Search Google Scholar
    • Export Citation
  • XuZ.Y.YangC.Y.YangM.X.1995Studies on the double fertilization and embryogeny in Dendrobium candidum Wall. ex LindlJournal of Tropical and Subtropical Botany (China)36064

    • Search Google Scholar
    • Export Citation
  • YeX.L.ChengS.J.1988Morphology of immature seeds and development in vitro of Dendrobium candidumActa Bot. Yunnanica10285290

  • YeX.L.GuoJ.Y.1995Female gametophyte development and embryogeny in Cymbium sinense (Andr.) WilldJournal of Tropical and Subtropical Botany (China)35458

    • Search Google Scholar
    • Export Citation
  • YeX.L.ZeeS.Y.YeungE.C.1997Suspensor development in the nun orchid, Phaius tankervilliaeInternational Journal of Plant Sciences158704712

    • Search Google Scholar
    • Export Citation
  • YeungE.C.ZeeS.Y.YeX.L.1994Embryology of Cymbidium sinense: Ovule developmentPhytomorphology445563

  • ZhaoP.WangW.FengF.S.WuF.YangZ.Q.WangW.J.2007High-frequency shoot regeneration through transverse thin cell layer culture in Dendrobium candidum Wall Ex LindlPlant Cell Tissue Organ. Cult.90131139

    • Search Google Scholar
    • Export Citation
  • ZhaoP.WuF.FengF.S.WangW.J.2008Protocorm-like body (PLB) formation and plant regeneration from the callus culture of Dendrobium candidum Wall ex LindlIn Vitro Cell. Dev. Biol. Plant44178185

    • Search Google Scholar
    • Export Citation
  • ZimmermanJ.L.1993Somatic embryogenesis: A model for early development in higher plantsPlant Cell514111423

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