Genetic Diversity Analysis of Nine Narcissus Based on Morphological Characteristics and Random Amplified Polymorphic DNA Markers

in HortScience

Genetic diversity of Narcissus was systematically studied on both morphological and molecular levels. Twenty-four characteristics of nine narcissi were observed and their differences evaluated by clustering method. The results showed that nine narcissi can be divided into two subclusters: one comprised by Narcissus pseudonarcissus, the other by Chinese Narcissus. The morphological diversity among five cultivars of N. pseudonarcissus is higher than that among four ecotypes of Chinese Narcissus (Narcissus tazetta var. chinensis). There are seven morphological characteristics in N. pseudonarcissus presenting obvious variations with coefficients from 33.33% to 91.67%. Only five morphological characteristics in Chinese Narcissus present certain variations with coefficients from 37.04% to 51.79%. On DNA level, two clusters are distantly related too. Based on the random amplified polymorphic DNA (RAPD) markers, 13 out of 40 random primers yielded scorable polymorphisms between samples. Wide variations in banding profiles between cultivars or between ecotypes were observed with nearly every primer tested. Among 95 band positions that were scored for all the 9 narcissi, 81 are polymorphic (85.26%). Cluster analysis of the calculated similarity matrix revealed that the genetic diversity between these individuals within the same section is low. However, the genetic diversity between two sections is obviously higher. Taken together, the methods combined morphological characteristics and RAPD technique allow a deep evaluation of the variation of Narcissus on both section level and cultivar/ecotype level.

Contributor Notes

This research was supported by Shanghai Chongming Project (grant 13391912504) and the Key Science and Technology Foundation (grant 13391901002) from Science and Technology Commission of Shanghai Municipality, Shanghai, China.

Corresponding author. E-mail: dqtang@sjtu.edu.cn.

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    Flower color of nine narcissi. (A) ‘Shangnong Wanxia’, (B) ‘Shangnong Zaochun’, (C) ‘Shangnong Ruhuang’, (D) ‘Shangnong Dieying’, (E) ‘Shangnong Hongying’, (F) Zhangzhou ‘Yulinglong’, (G) Zhangzhou ‘Jinzhanyintai’, (H) Putuo ‘Jinzhanyintai’, (I) Chongming ‘Jinzhanyintai’.

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    Dendrograms of clustering analysis based on (A) agro-morphological characteristics of vegetative organs, (B) morphological characteristics of inflorescence and flower, and (C) total 24 morphological characteristics.

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    The gel photographs obtained with 13 random primers. Note for Lanes: M, DL5,000 DNA marker; 1, ‘Shangnong Wanxia’; 2, ‘Shangnong Zaochun’; 3, ‘Shangnong Ruhuang’; 4, ‘Shangnong Dieying’; 5, ‘Shangnong Hongying’; 6, Zhangzhou ‘YuLinglong’; 7, Zhangzhou ‘Jinzhanyintai’; 8, Putuo ‘Jinzhanyintai’; 9, Chongming ‘Jinzhanyintai’.

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    Dendrogram based on random amplified polymorphic DNA (RAPD) markers illustrating the genetic relationship among the analyzed narcissi.

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