USVL-380, a Zucchini yellow mosaic virus-resistant Watermelon Breeding Line

in HortScience

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Contributor Notes

Corresponding author. E-mail: amnon.levi@ars.usda.gov.

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    Pedigree of USVL-380 showing the route of incorporating the Zucchini yellow mosaic virus resistance gene locus into the genome of the watermelon cultivar Charleston Gray (Citrullus lanatus var. lanatus).

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    Genotyping of plants throughout the breeding process of USVL-380 using the CAPS-2 marker for the eIF4E allele. Genotyping of CAPS-2 was performed using the primer pair KL08-03, 5′-AAAGCTACACCCACGGAAGA and KL08-04, 5′-CTCCAGAACTCCTCGACAGTAG and by digestion of their polymerase chain reactions amplicon with restriction enzyme Pas I (as described by Harris et al., 2009; Ling et al., 2009). From left to right, lanes 1 (P) is the Zucchini yellow mosaic virus (ZYMV)-resistant PI 595203 parent (211 bp fragment). Lane 2 (N) is the susceptible watermelon cultivar Charleston Gray (268 bp fragment). Lane 3 is the F1 hybrid derived from crossing PI 595203 and ‘New Hampshire Midget’. Lanes 4–12 are susceptible (S) and resistant (R) F2 progeny. The R progeny are homozygous for the eIF4E allele (211 bp fragment) derived from the ZYMV-resistant PI 595203, whereas the ZYMV-S plants are heterozygous for the eIF4E allele, or homozygous for the eIF4E allele derived from the parent cultivar.

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    USVL-380 fruits harvested in the field in Charleston, SC (Summer 2014).

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    USVL-380 fruits harvested in the field in Charleston, SC (Summer 2015).

Article References

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