Histological and Biochemical Traits of Chilling-injured Pulp Tissues as Affected by Cold Storage of Mango Fruit

in HortScience

Green mango (Mangifera indica L.) ‘Nam Doc Mai See Thong’ fruit were dipped in 2-chloroethylphosphonic acid solution (50 ppm) for 5 minutes, kept at 25 °C for 3 days, cold stored at 5 °C for 35 days and then transferred to 25 °C for 7 days. The skin color of the cold-stored fruit partly changed to dark-brown with surface depression. In addition, desiccated white-corky pulp tissues developed mainly along to the dark-brownish skin. Histological and biochemical analyses revealed that the formation of white-corky pulp tissues was correlated with starch accumulation in the hypodermal cells. Cell wall polymers of the white-corky pulp tissues were characterized by both a lower amount of solubilized pectins and higher amount of hemicelluloses than those of normally ripened (NR) tissues. The highest fatty acid unsaturation was observed in the NR pulps under chilling conditions followed by the white-corky pulp tissues under chilling conditions and the NR tissues without chilling. These results suggested that the disordered membrane caused by chilling inhibited the subsequent cascade of secondary reactions, such as the cell wall degradation. The skin damage derived from chilling injury (CI) is a direct factor inducing abnormal desiccation in the adjacent pulp, resulting in the formation of white-corky pulp tissues.

Contributor Notes

This study was supported by CMU Short Term Research Fellowships in Overseas and MEXT/JSPS KAKENHI Grant Number 22580026.The authors acknowledge Kenji Takahashi, Midori Sugawara, and Rieko Kawamata for their excellent technical assistance.

Corresponding author. E-mail: nisizawa@tds1.tr.yamagata-u.ac.jp.

  • AntunesM.D.C.SfakiotakisE.M.2008Changes in fatty acid composition and electrolyte leakage of ‘Hayward’ kiwifruit during storage at different temperaturesFood Chem.110891896

    • Search Google Scholar
    • Export Citation
  • BandyopadhyayC.GholapA.S.1973Changes in fatty acids in ripening mango pulp (variety Alphonso)J. Agr. Food Chem.21496497

  • BlumenkrantzN.Asboe-HansenG.1973New method for quantitative determination of microgram quantities of uronic acidsAnal. Biochem.54484489

    • Search Google Scholar
    • Export Citation
  • BurondkarM.M.JadhavB.B.ChettiM.B.2009Effect of plant growth regulators, polyamine and nutrients on fruit quality and occurrence of spongy tissue in Alphonso mangoActa Hort.820689696

    • Search Google Scholar
    • Export Citation
  • ChaplinG.R.ColeS.P.LandriganM.NuevoP.A.LamP.F.GrahamD.1991Chilling injury and storage of mango (Mangifera indica L.) fruit held under low temperatureActa Hort.291461471

    • Search Google Scholar
    • Export Citation
  • ChhatparH.S.MattooA.K.ModiV.V.1971Biochemical studies on chilling injury in mangoesPhytochemistry1010071009

  • ChomchalowN.SongkhlaP.N.2008Thai mango export: A slow-but-sustainable developmentAU J.T.1218

  • ChourasiaA.SaneV.A.NathP.2006Differential expression of pectate lyase during ethylene-induced postharvest softening of mango (Mangifera indica var. Dashehari)Physiol. Plant.128546555

    • Search Google Scholar
    • Export Citation
  • DingZ.-S.TianS.-P.ZhengX.-L.ZhouZ.-W.XuY.2007Responses of reactive oxygen metabolism and quality in mango fruit to exogenous oxalic acid or salicylic acid under chilling temperature stressPhysiol. Plant.130112127

    • Search Google Scholar
    • Export Citation
  • DuboisM.GillesK.A.HamiltonJ.K.RebersP.A.SmithF.1956Colorimetric method for determination of sugars and related substancesAnal. Chem.28350356

    • Search Google Scholar
    • Export Citation
  • ForneyC.F.1990Ripening and solar exposure alter polar lipid fatty acid compostition of ‘Honey Dew’ muskmelonsHortScience2512621264

  • González-AguilarG.A.FortizJ.CruzR.WangC.Y.2000Methyl jasmonate reduces chilling injury and maintains postharvest quality of mango fruitJ. Agr. Food Chem.48515519

    • Search Google Scholar
    • Export Citation
  • GuptaN.JainS.K.2014Storage behaviour of mango as affected by post harvest application of plant extracts and storage conditionsJ. Food Sci. Technol.5124992507

    • Search Google Scholar
    • Export Citation
  • HanJ.TianS.-P.MengX.-H.DingZ.-S.2006Response of physiologic metabolism and cell wall structures in mango fruit to exogenous methyl salicylate under low-temperature stressPhysiol. Plant.128125133

    • Search Google Scholar
    • Export Citation
  • JacobiK.K.WongL.S.1992Quality of ‘Kensington’ mango (Mangifera indica Linn.) following hot water and vapour-heat treatmentsPostharvest Biol. Technol.4349359

    • Search Google Scholar
    • Export Citation
  • JanaveM.T.2009Profile of peroxidase isoforms influenced by spongy tissue disorder in Alphonso mango (Mangifera indica L.) fruitsActa Physiol. Plant.3111761184

    • Search Google Scholar
    • Export Citation
  • JanaveM.T.SharmaA.2008Spongy tissue development in Alphonso mango: Association with Staphylococcus xylosusEur. J. Plant Pathol.122335348

    • Search Google Scholar
    • Export Citation
  • KaneO.BouletM.CastaigneF.1982Effect of chilling-injury on texture and fungal rot of mangoes (Mangifera indica L.)J. Food Sci.47992995

    • Search Google Scholar
    • Export Citation
  • KaneO.MarcellinP.MazliakP.1978Incidence of ripening and chilling injury on the oxidative activities and fatty acid compositions of the mitochondria from mango fruitsPlant Physiol.61634638

    • Search Google Scholar
    • Export Citation
  • KatrodiaJ.S.1988Spongy tissue in mango—Causes and control measuresActa Hort.231814826

  • KrishnamurthyS.1980Internal breakdown during ripening of Alphonso mango (Mangifera indica Linn.) in relation to specific gravity of the fruitJ. Food Sci. Technol.17198199

    • Search Google Scholar
    • Export Citation
  • LimaL.C.O.ChitarraA.B.ChitarraM.I.F.2001Changes in amylase activity starch and sugars contents in mango fruits pulp cv. Tommy Atkins with spongy tissue. BrazilArch. Biol. Technol.445962

    • Search Google Scholar
    • Export Citation
  • LizadaM.C.1991Postharvest physiology of the mango - A reviewActa Hort.291437453

  • MachhindraT.J.SharmaA.2008Spongy tissue development in Alphonso mango: Association with Staphylococcus xylosusEur. J. Plant Pathol.122335348

    • Search Google Scholar
    • Export Citation
  • McCollumT.G.D’AquinoS.McDonaldR.E.1993Heat treatment inhibits mango chilling injuryHortScience28197198

  • MedlicottA.P.SigristJ.M.M.SyO.1990Ripening of mangoes following low-temperature storageJ. Amer. Soc. Hort. Sci.115430434

  • MiguelA.C.A.DuriganJ.F.FerraudoA.S.2011Multivariate analysis that can be used to evaluate the chilling injury in mangoes cv. Tommy Atkins (in Portoguese)Rev. Bras. Fruticu.33371376

    • Search Google Scholar
    • Export Citation
  • MitchamE.J.McDonaldR.E.1992Cell wall modification during ripening of ‘Keitt’ and ‘Tommy Atkins’ mango fruitJ. Amer. Soc. Hort. Sci.117919924

    • Search Google Scholar
    • Export Citation
  • MohammedM.A.BrechtJ.K.2002Reduction of chilling injury in ‘Tommy Atkins’ mangoes during ripeningSci. Hort.95297308

  • MontalvoE.GarcíaH.S.TovarB.MataM.2007Application of exogenous ethylene on postharvest ripening of refrigerated ‘Ataulfo’ mangoesLWT4014661472

    • Search Google Scholar
    • Export Citation
  • NagamaniJ.E.ShivashankaraK.S.RoyT.K.2010Role of oxidative stress and the activity of ethylene biosynthetic enzymes on the formation of spongy tissue in ‘Alphonso’ mangoJ. Food Sci. Technol.47295299

    • Search Google Scholar
    • Export Citation
  • NairS.SinghZ.2004Chilling injury in mango fruit in relation to biosynthesis of free polyaminesJ. Hortic. Sci. Biotechnol.79515522

  • NairS.SinghZ.TanS.C.2001Heat treatments affect development chilling injury, respiration, ethylene production and fruit quality of mangoActa Hort.553549550

    • Search Google Scholar
    • Export Citation
  • NishizawaT.ItoA.MotomuraY.ItoM.TogashiM.2000Changes in fruit quality as influenced by shading of netted melon plants (Cucumis melo L. ‘Andesu’ and ‘Luster’)J. Jpn. Soc. Hort. Sci.69563569

    • Search Google Scholar
    • Export Citation
  • NishizawaT.ItoA.2007Analysis of cell wall polysaccharides during storage of a local melon accession ‘Wasada-uri’ compared to the melon cultivar ‘Prince’J. Hort. Sci. Biotechnol.82227234

    • Search Google Scholar
    • Export Citation
  • NishizawaT.NagasawaS.RetamalesJ.B.LavínA.MotomuraY.2002Comparison of cell wall components between Fragaria X ananassa and Fragaria chiloensis grown in ChileJ. Hort. Sci. Biotechnol.77404410

    • Search Google Scholar
    • Export Citation
  • PesisE.AharoniD.AharonZ.Ben-ArieR.AharoniN.FuchsY.2000Modified atmosphere and modified humidity packaging alleviates chilling injury symptoms in mango fruitPostharvest Biol. Technol.1993101

    • Search Google Scholar
    • Export Citation
  • PratesJ.A.M.AlfaiaC.M.AlvesS.P.BessaR.J.B.2011Fatty acids p. 137–161. In: L.M.L. Nollet and F. Toldra (eds.). Handbook of analysis of edible animal by-products. CRC Press New York

  • RahmanM.M.RahmanM.M.AbsarN.AhsanM.A.2011Correlation of carbohydrate content with the changes in amylase, invertase and β-galactosidase activity of ripe mango pulp during storage under different temperaturesBangladesh J. Sci. Ind. Res.46443446

    • Search Google Scholar
    • Export Citation
  • RajaM.E.2009Investigation on causes and correction of spongy tissue in Alphonso mango (Mangifera indica L.)Acta Hort.820697706

  • RavindraV.ShivashankarS.2004Spongy tissue in Alphonso mango – significance of in situ seed germination eventsCurr. Sci. India8710451049

    • Search Google Scholar
    • Export Citation
  • RavindraV.ShivashankarS.2006Spongy tissue in Alphonso mango. II. A key evidence for the causative role of seedCurr. Sci. India9117121714

    • Search Google Scholar
    • Export Citation
  • RoseJ.K.C.HadfieldK.A.LabavitchJ.M.BennettA.B.1998Temporal sequence of cell wall disassembly in rapidly ripening melon fruitPlant Physiol.117345361

    • Search Google Scholar
    • Export Citation
  • SaranwongS.SornsrivichaiJ.KawanoS.2004Prediction of ripe-stage eating quality of mango fruit from its harvest quality measured nondestructively by near infrared spectroscopyPostharvest Biol. Technol.31137145

    • Search Google Scholar
    • Export Citation
  • ShewfeltR.L.del RosarioB.A.2000The role of lipid peroxidation in storage disorders of fresh fruits and vegetablesHortScience21951956

  • ShivashankaraK.S.IsobeS.Al-HaqM.I.TakenakaM.ShiinaT.2004Fruit antioxidant activity, ascorbic acid, total phenol, quercetin, and carotene of Irwin mango fruits stored at low temperature after high electric field pretreatmentJ. Agr. Food Chem.5212811286

    • Search Google Scholar
    • Export Citation
  • ShivashankarS.RavindraV.LouisL.2007Biochemical changes in seed and mesocarp of mango (Mangifera indica L.) cv. ‘Alphonso’ and their significance during the development of spongy tissueJ. Hort. Sci. Biotechnol.823540

    • Search Google Scholar
    • Export Citation
  • SimãoR.A.SilvaA.P.F.B.PeroniF.H.G.NascimentoJ.R.O.LouroR.P.LajoloF.M.CordenunsiB.R.2008Mango starch degradation. I. a microscopic view of the granule during ripeningJ. Agr. Food Chem.5674107415

    • Search Google Scholar
    • Export Citation
  • SinghZ.JanesJ.2001Effects of postharvest application of ethephon on fruit ripening, quality and shelf life of mango under modified atmosphere packagingActa Hort.553599602

    • Search Google Scholar
    • Export Citation
  • SinghZ.SinghR.K.SaneV.A.NathP.2013Mango - postharvest biology and biotechnologyCrit. Rev. Plant Sci.32217236

  • SivakumarD.JiangY.YahiaE.M.2011Maintaining mango (Mangifera indica L.) fruit quality during the export chainFood Res. Intl.4412541263

    • Search Google Scholar
    • Export Citation
  • ThinhD.-C.UthaibutraJ.JoomwongA.2013Effect of storage temperatures on ripening behavior and quality change of Vietnamese mango cv. Cat Hoa LocIJBTR31930

    • Search Google Scholar
    • Export Citation
  • ThomasP.JoshiM.R.1988Reduction of chilling injury in ripe Alphonso mango fruit in cold storage by temperature conditioningIntl. J. Food Sci. Technol.23447455

    • Search Google Scholar
    • Export Citation
  • ThomasP.OkeM.S.1983Improvement in quality and storage of ‘Alphonso’ mangoes by cold adaptationSci. Hort.19257262

  • VasanthaiahH.K.N.RavishankarK.V.ShivashankaraK.S.AnandL.NarayanaswamyP.MukundaG.PrasadT.G.2006Cloning and characterization of differentially expressed genes of internal breakdown in mango fruit (Mangifera indica)J. Plant Physiol.163671679

    • Search Google Scholar
    • Export Citation
  • WangB.WangJ.LiangH.YiJ.ZhangJ.LinL.WuY.FengX.CaoJ.JiangW.2008Reduced chilling injury in mango fruit by 2,4-dichlorophenoxyacetic acid and the antioxidant responsePostharvest Biol. Technol.48172181

    • Search Google Scholar
    • Export Citation
  • YashodaH.M.PrabhaT.N.TharanathanR.N.2006Mango ripening: Changes in cell wall constituents in relation to textural softeningJ. Sci. Food Agr.86713721

    • Search Google Scholar
    • Export Citation
  • ZaharahS.S.SinghZ.2011Mode of action of nitric oxide in inhibiting ethylene biosynthesis and fruit softening during ripening and cool storage of ‘Kensington Pride’ mangoPostharvest Biol. Technol.62258266

    • Search Google Scholar
    • Export Citation
  • ZhangC.TianS.2009Crucial contribution of membrane lipids’ unsaturation to acquisition of chilling-tolerance in peach fruit stored at 0°CFood Chem.115405411

    • Search Google Scholar
    • Export Citation
All Time Past Year Past 30 Days
Abstract Views 112 112 9
Full Text Views 71 71 0
PDF Downloads 5 5 0