Characterization and Identification of Spanish Olive Germplasm by Means of RAPD Markers

in HortScience

Thirteen randomly amplified polymorphic DNA (RAPD) primers were assayed in 82 Spanish olive cultivars of economical interest. A total of 82 bands were scored giving an average of 6.3 bands per primer. A total of 4 (OPA-01) to 10 bands (OPA-19) was amplified, while the number of polymorphic fragments ranged from 2 (OPK-07) to 9 (OPA-19) with a mean of 5.7 polymorphic bands per primer. A total of 89% of the amplification products (73 bands) were polymorphic. The 13 primers yielded 184 banding patterns (14.9 per primer). The number of banding patterns per primer ranged from 4 (OPK-07) to 39 (OPA-19). Fifty-three unique banding patterns were found, the majority of them resulted from different combinations of polymorphic bands. The combination of only five primers OPA-19, OPF-06, OPX-01, OPX-03, and OPI-12, allowed identification of all the cultivars. Seventy-four cultivars (90%) were identified only by the combination of the first four primers. The addition of the fifth primer (OPI-12) was necessary for the identification of the eight remaining cultivars (10%). The ordination of the primers according to their practical discriminating capacity in this study was: OPA-19 > OPF-06 > OPX-01 > OPX-03 > OPI-12. Hence RAPD markers are recommended for olive fingerprinting in order to generate a database for olive cultivar identification.

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