Media Composition and Light Affect Storability and Poststorage Recovery of Micropropagated Hosta Plantlets

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  • 1 Department of Horticulture, Clemson University, Clemson, SC 29634-0375
  • 2 Department of Horticulture, Clemson University, Clemson, SC 29634-0375
  • 3 Department of Agricultural and Biological Engineering, Clemson University, Clemson, SC 29634-0357

Hosta (Hosta tokudama Makeawa `Newberry Gold') plantlets were micropropagated photoautotrophically (without sucrose in medium) or photomixotrophically (with 2% sucrose in medium) for 3 weeks at 23 °C under 80 μmol·m-2·s-1 photosynthetic photon flux (PPF) prior to long-term storage. Plantlets were stored for 4, 8, or 12 weeks at 5, 10, or 22 °C in darkness or under white (400-800 nm), blue (400-500 nm), or red (600-700 nm) light at or near light compensation points. Illumination during storage was necessary to maintain dry weight and regrowth potentials of plantlets in vitro, but light quality had no effect on these parameters. All photoautotrophic plantlets stored in darkness were of poor quality at the time of removal from storage and died when transferred to the greenhouse. Dark-stored photomixotrophic plantlets survived storage for 12 weeks at 5 °C, but declined in appearance (visual quality) as the storage duration increased. Decline in visual quality was greater when plantlets were stored at 10 and 22 °C. Leaf dry weight of illuminated plantlets increased and percentage of leaf yellowing decreased as storage temperature increased. Recovery of illuminated plantlets from photomixotrophic storage was best when plantlets were stored at 22 °C. These plantlets were characterized by increased visual quality (color and form) and increased dry weight compared with those in other treatments. After 60 days in the greenhouse, the dry weight of these plantlets was similar for 4-, 8-, and 12-week storage durations, indicating flexibility in storage time if specific light and temperature provisions are met.

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To whom reprint requests should be addressed. Current address: 2199 South Rock Road, Indian River Research and Education Center, University of Florida, Fort Pierce, FL 34945. E-mail address: Sbwilson@gnv.ifas.ufl.eduCurrent address: Department of Agricultural and Biological Engineering, Pennsylvania State University, University Park, PA 16802.
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