Randomly Amplified Polymorphic DNA Markers Linked to Fusarium Wilt Resistance in Diverse Melons

in HortScience

Three randomly amplified polymorphic DNA (RAPD) markers (E07, G17, and 596) linked to the Fom-2 gene, which confers resistance to race 0 and 1 of Fusarium oxysporum f. sp. melonis, were evaluated by RAPD-polymerase chain reaction for their linkage to Fusarium wilt resistance/susceptibility in diverse melon cultigens (48 resistant, 41 susceptible). Primer 596 was identified in the multiple disease-resistant breeding line MR-1, whereas E07 and G17 were identified in the susceptible `Vedrantais'. The RAPD markers E07 (1.25 kb) and G17 (1.05 kb) correctly matched phenotypes in 88% and 81% of the cultigens. The validity of the RAPD scores was verified by Southern hybridization analysis for sequence homology and bulked segregant analysis of a selected cross population for the linkage. These results will facilitate the introgression of resistance genes into susceptible lines from multiple sources in marker-assisted selection.

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Contributor Notes

To whom reprint requests should be addressed. X.Y. Zheng; E-mail address: xi-zheng@tamu.edu. Fax: (956) 969-5620.Current address: Sakata Seed America, Inc., P.O. Box 1118, Lehigh Acres, FL 33970-1118.

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