Paternity Analysis in Higher Polyploids

in HortScience

The breeding of new sweetpotato varieties is a highly inefficient process, confounded by incompatibility, poor fertility, open-pollination, and its hexaploid nature. Upwards of 12 to 20 lines are currently combined in open-pollinated nurseries based on good horticultural characteristics. Most progeny after several years of selection can be traced back to just three or four maternal lines. A method that would identify the paternal parent of superior progeny would enable breeders the ability to combine parents that exhibit superior combining ability in more-efficient, smaller nurseries. The objective of this work is to explore by means of computer simulation the application of genealogy reconstruction techniques on hexaploid individuals with PCR-based data. The progeny obtained on each female parent is fractionally assigned to each male with non zero exclusion probability proportional to its paternity likelihood. Computer simulations show that at least five different alleles per loci are needed to reach a reasonable discriminatory level. Also, the number of loci scored should not be less than 20. An increment in the number of alleles or loci increases the discriminatory power; but, the number of alleles produces a far more important effect than the number of loci.

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