474 PB 370 EFFECT OF PRIOR INOCULATION OF LEAVES OF DRY BEANS WITH THE COMMON BLIGHT PATHOGEN ON THE DISEASE REACTION OF SUBSEQUENTLY INOCULATED LEAVES AND PODS AND TEE REACTION OF DETACHED VERSUS ATTACHED PODS
Breeding for resistance is a major method to control the common bacterial blight disease caused by Xanthomonas campestris pv phaseoli (Xcp) in common bean (Phaseolus vulgaris L.). It is necessary to determine if prior inoculation of the first trifoliolate leaf with Xcp will influence the subsequent reaction in other plant parts through induced resistance. It is difficult to get an accurate estimate of heritability (H) of disease reaction in pods since environment (E) greatly affects the H estimate if flowering occurs over extended time periods. Thus the disease reaction in attached pods vs detached pods was also observed. Four common bean lines were used in a split plot design with two replications. Two bacterial strains were used for inoculations. Two growth chambers were used as replicates. The first trifoliolate leaves, later developed leaves and attached pods and detached pods were inoculated. No effect of prior inoculation on the disease reactions of subsequently inoculated leaves and pods were observed indicating that the different plant parts can be inoculated at different times. Detached and attached pods showed similar disease symptoms. The former may be used to reduce E variance and improve H estimates.
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