046 COMPARATIVE GROWTH RESPONSES AND COSTS UTILIZING TRADITIONAL AND AUTOMATED TISSUE CULTURE SYSTEMS

in HortScience

Comparative tests were conducted to determine the influence of the culture vessel size and medium volume on the growth rates of shoot tips of peas (Pisum sativum cv. `Wando'), lettuce (Lactuca salvia) Kidney beans (Phaseolus vulgaris). Culture vessels employed included: culture tubes, baby food jars, Magenta rectangular containers, 1 -pint Mason jars, 1 -quart Mason jars, l-quart Mason jars employed with an automated plant culture system (APCS), 1/2-gallon Mason jars with an APCS, BioSafe containers with an APCS, and mega-culture chambers with an APCS. The APCS consisted of a peristaltic pump, media reservoir containing 1 liter of nutrient medium, and a culture chamber (<925 mm3). High positive correlations occurred comparing culture weight, leaf length and plant height with culture chamber volume, media volume and culture chamber height. APCSs consistently gave higher growth rates and exhibition of mature morphogenetic responses such as flowering and fruiting than growing plants on agar culture systems. Cost analysis comparing APCSs and conventional tissue culture systems is presented.

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