029 COMPARISON OF ADVENTITIOUS REGENERATION SCHEMES IN WHITE ASH

in HortScience

White ash has been adventitiously regenerated via organogenesis, somatic embryogenesis, and nodule culture. Explant source genotype, plant growth regulator type and concentration affected the type and/or frequency of regeneration observed. Organogenesis was obtained only when thidiazuron was added to the medium and nodules formed only in liquid culture after exposure to BA. Somatic embryos formed when explants were exposed to 2,4-D regardless of cytokinin used. Although large numbers of somatic embryos and nodules may be obtained through liquid suspension cultures, few plantlets were recovered compared to shoot organogenesis. Elongated adventitious shoots elongated and epicotyls from germinated somatic embryos rooted easily in vitro or under intermittent mist and were then acclimatized to the greenhouse and planted in the field.

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