DIFFERENTIAL HYDRATION AND ELEMENTAL ABSORPTION OF TWO POLYACRYLAMIDE GELS

in HortScience

Hydration and elemental absorption of two commercially-available polyacrylamide gels (A and B) were studied in response to a 24-hr soak time in Hoagland's solution concentrations of either 2X, 1X, 0.5X, 0.25X, 0.125X or 0X (deionized water). Elemental absorption of gel specimens was observed and analyzed within the gel matrix on a Philips CM12S STEM equipped with an EDAX 9800 plus EDS unit for micro x-ray analysis. Thick sections were cut on dry glass knives using an RMC MT6000 ultramicrotome. Surface analysis of bulk specimens was made with an AMR 1000A SEM plus PGT1000 EDS unit. Overall, gel hydration decreased quadratically as solution concentration increased linearly; however, hydration for gel A was generally greater than for gel B. Surface analysis of gel samples revealed the presence Ca, K, P, S, Fe, and Zn for both gels. An analysis within the matrix of gel B revealed the presence of Ca, K, P, S, Fe, and Zn; however, an analysis within the matrix of gel A revealed the presence of Zn, and Fe only. The increased absorptive capacity of gel A appeared to be coupled to reduced migration of salts into the gel matrix.

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