Rapid senescence of day lily flowers (Hemerocallis sp. cv. Cradle Song) has been shown to be associated with a rapid disappearance of proteins. Senescence was significantly delayed by pulsing developing flowers with cycloheximide, an inhibitor of protein synthesis. A cDNA library prepared from mRNA extracted from flowers in the very early stages of senescence was probed with mRNA from flowers at different stages of opening and senescence. Characterization of senescence-specific clones, and implications for the control of senescence in this non-climacteric flower will be discussed.
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