IN VITRO ORGANOGENESIS OF `BEAUREGARD' SWEETPOTATO

in HortScience

Methodology is presented for organogenesis of `Beauregard sweetpotato, a cultivar released in 1987 that is rapidly increasing in commercial use in the U.S. Regeneration of `Beauregard' sweetpotato plantlets was obtained when complete leaves and 10 mm internode explants were cultured in liquid and solid media respectively over a period of 8 weeks. Leaves in liquid Murashige and Skoog medium containing 2 mg/l of IAA placed on a shaker under dark conditions produced white callus at the cut end of the petiole and roots underneath the callus in 4 weeks. Leaves were subsequently transferred to MS medium containing 500 mg/l of Chlorocholine chloride (CCC) and 0, 1, 5 and 10 mg/l of BAP for 4 more weeks. Shoots were regenerated from callus using 1 mg/l of BAP.

The effect of NAA auxin and various concentrations of the cytokinin Thidiazuron on internodes was examined under 16 hr. light and 8 hr dark photoperiod using MS solid medium. Explants on 0.05 mg/l NAA alone produced roots and shoots. The most plantlets however, were regenerated using 0.05 mg/l of NAA and 0.01 mg/l Thidiazuron. Regeneration of plants from leaves and internodes may be a useful system for a clean and rapid propagation of `Beauregard' sweetpotato.

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