University of Illinois, Department of Horticulture, Plant Science Lab, 1201 S. Dorner, Urbana, IL.
Calli were initiated from immature inflorescences of selected cultivars of Hosta in the light on Hosta initiation medium (Carolina Biological Supply Company, 1986). Three cultivars, Francee, Birchwood Park's Gold, and Wide Brim Sum & Substance, produced microshoots. The calli were compact and green in color. The highest percentage of callus formation occurred with the Francee cultivar. Nakaiana, Golden Edger, Golden Scepter, Obscura, Sum & Substance, and Shade Fanfare produced only calli. The calli were transferred to modified Murashige and Skoog salts. The media containing 5 × 5 factorial combinations of NAA and BA (0.0, 0.1, 0.5, 1.0, or 2.0 mg/l). The results show that media with NAA at 1.0 and 2.0 mg/l in combination with BA from 0.5 to 2.0 mg/l produced the highest number of microshoots per explant.
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