We have used isozyme techniques (SGE) to assess variation and begin construction of a genetic map of the Asparagus officinalis genome. Isozyme extraction buffers, electrophoretic buffer systems, and isozyme stability during storage were evaluated. Isozyme expression under different environmental conditions was also examined. Thirty-four enzymes were evaluated for their usefulness as genetic markers in A. officinalis. Of these 34, 13 had sufficient activity and resolution on the gels for isozyme analysis. Of the 13 enzyme systems resolved, polymorphisms were observed in aconitase, endopeptidase, malate dehydrogenase, phosphoglucomutase, and shikimate dehydrogenase. Segregation of putative alleles is presented for ACON, END, MDH, PGM and SKDH isozymes. Co-segregation data showed linkage between a SKDH locus and a PGM locus. The isozyme analysis also included Asparagus densiflorus `Sprengeri' and revealed that aspartate aminotransaminase, endopeptidase, and triosephosphate isomerase would be potentially useful for verification of cell fusion products between the two species.
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