EFFECTS OF MACRONUTRIENT CONTENT OF CELL LAYER MEDIA ON SURVIVAL, DIAMETER AND PLATING EFFICIENCY OF `RUSSET BURBANK' POTATO PROTOPLASTS.

in HortScience

In order to determine the appropriate nutritional conditions for survival, mesophyll protoplasts cv. “Russet Burbank” were cultured in three cell layer media. Each contained the MS macronutrients at either 0, 1 or 4 times the concentration recommended by Shepard (1980). During the 14 day culture period, cells were evaluated and counted, diameter determined and plating efficiency calculated. Results will be discussed.

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