In order to determine the appropriate nutritional conditions for survival, mesophyll protoplasts cv. “Russet Burbank” were cultured in three cell layer media. Each contained the MS macronutrients at either 0, 1 or 4 times the concentration recommended by Shepard (1980). During the 14 day culture period, cells were evaluated and counted, diameter determined and plating efficiency calculated. Results will be discussed.
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