Isozyme Verification of Interspecific Hybrids of Cuphea

in HortScience
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  • 1 U.S. Water Conservation Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Phoenix, AZ 85040

Five interspecific Cuphea hybrids were examined for isozyme banding patterns. In three of the five hybrids (C. viscosissima × C. lutea, C. ignea × C. angustifolia, and C. lanceolata × C. viscosissima), F1 plants could be distinguished from either parental species. Phosphoglucomutase and 6-phosphogluconic dehydrogenase enzyme stains produced distinct F1 band patterns for all three hybrids. Phosphoglucose isomerase and shikimate dehydrogenase enzyme stains produced distinct F1 band patterns for C. viscosissima × C. lutea and C. lanceolata× C. viscosissima, respectively. For the C. lanceolata × C. viscosissima hybrid. the banding patterns were used to identify 32 selfs among 161 putative F1 plants.

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